Conservation Genetics Resources

, Volume 6, Issue 4, pp 837–839 | Cite as

Oviductal eggs from road-kill turtles provide a novel source of DNA for population studies of the Alabama red-bellied turtle

  • Elizabeth E. Hieb
  • David H. Nelson
  • Ashley B. Morris
Technical Note
  • 108 Downloads

Abstract

Genetic analyses of the endangered Alabama red-bellied turtle (Pseudemys alabamensis) have the potential to answer questions regarding the species’ population dynamics and reproductive ecology. Here we used oviductal eggs from road-kill female turtles to obtain DNA from P. alabamensis specimens. Six microsatellite markers were successfully amplified in egg yolks/developing embryos. DNA taken from multiple eggs in the same clutch showed variation in allele sizes at multiple loci, indicating amplification of both maternally and paternally contributed alleles. These results introduce DNA extraction from oviductal eggs from road-kill mortalities as a novel approach for examining the reproductive habits and population dynamics of P. alabamensis and similar turtle species. This approach may be particularly effective in exploring mating patterns and monitoring population stability in endangered species where traditional sampling methods are difficult to perform.

Keywords

Conservation genetics Multiple paternity Pseudemys alabamensis Egg DNA 

References

  1. Baur G (1893) Notes on the classification and taxonomy of the Testudinata. Proc Am Philos Soc 31:210–225Google Scholar
  2. Hieb EE, Jackson TG, Nelson DH, Morris AB (2011) Characterization of eight polymorphic microsatellite loci for the endangered Alabama red-bellied turtle (Pseudemys alabamensis; Emydidae). Conserv Genet Resour 3(4):781–783CrossRefGoogle Scholar
  3. King TL, Julian SE (2004) Conservation of microsatellite DNA flanking sequence across 13 Emydid genera assayed with novel bog turtle (Glyptemys muhlengergii) loci. Conserv Genet 5:719–725CrossRefGoogle Scholar
  4. Leary CJ, Dobie JL, Mann TM, Floyd PS, Nelson DH (2008) Pseudemys alabamensis Baur 1893—Alabama red-bellied cooter, Alabama red-bellied turtle. Conservation Biology of Freshwater Turtles and Tortoises: A compilation project of the IUCN/SSC Tortoise and Freshwater Turtle Specialist Group. Chelonian Res Monogr 5: 019.1–019.9Google Scholar
  5. Nelson DH (2001) Population ecology of the Alabama redbelly turtle (Pseudemys alabamensis)—nesting survey. Final report to the Alabama Department Of Conservation and Natural Resources pp 46Google Scholar
  6. Nelson DH (2009) Annual report: the monitoring of roadside fencing and road-kill mortality to conserve Alabama red-bellied turtles (Pseudemys alabamensis) along the Mobile Bay Causeway. Alabama Department of Conservation and Natural Resources, Division of Wildlife and Freshwater Fisheries pp18Google Scholar
  7. Pearse DE, Avise JC (2001) Turtle mating systems: behavior, sperm storage, and genetic paternity. J Hered 92:205–211CrossRefGoogle Scholar
  8. Shamblin BM, Dodd MG, Williams KL, Frick MG, Bell RB, Nairn CJ (2011) Loggerhead turtle eggshells as a source of maternal nuclear genomic DNA for population genetic studies. Mol Ecol Resour 11:110–115PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Elizabeth E. Hieb
    • 1
  • David H. Nelson
    • 1
  • Ashley B. Morris
    • 2
  1. 1.Department of BiologyUniversity of South AlabamaMobileUSA
  2. 2.Department of BiologyMiddle Tennessee State UniversityMurfreesboroUSA

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