Conservation Genetics Resources

, Volume 6, Issue 4, pp 1031–1034 | Cite as

Isolation and characterization of polymorphic microsatellite loci for Siphamia tubifer Weber (Perciformes: Apogonidae)

  • Tilman J. AlpermannEmail author
  • Jörg Plieske
  • Ahmad O. Mal
  • Ofer Gon
  • Michael L. Berumen
Microsatellite Letters


The cardinalfish Siphamia tubifer has been selected as a model for the study of genetic connectivity in reef-associated fishes among marine-protected-areas in Socotra Island in the northwestern Indian Ocean (part of the Socotra Archipelago, a UNESCO World Heritage Site since 2008). Twenty-six novel microsatellite markers are described for S. tubifer and are now available for studies on its genetic population structure. In a population sample from Socotra Island, the newly developed markers possessed between three and 20 alleles. Expected and observed heterozygosity ranged from 0.56–0.96 to 0.55–0.95, respectively. The markers did not show deviations from Hardy–Weinberg equilibrium and were not in linkage disequilibrium.


Siphamia tubifer Microsatellites Population genetics Connectivity Marine protected areas 



This study was funded by the research funding program LOEWELandes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz of Hesse’s Ministry of Higher Education, Research, and the Arts and by the scientific research cooperation between King Abdulaziz University (KAU), Faculty of Marine Sciences (FMS), Jeddah, Saudi Arabia, and the Senckenberg Research Institute (SRI), Frankfurt, Germany, in the framework of the Red Sea Biodiversity Project (KAU Grant No. “D/1/432-DSR”). Friedhelm Krupp (Senckenberg Research Institute and Natural History Museum Frankfurt (SF) and Qatar Museum Authority) and Uwe Zajonz (Biodiversity and Climate Research Centre (BiK-F) and SF) are acknowledged for promoting this study as part of the BiK-F research program on tropical marine ecosystems. Mohamed Ahmer and Fouad Naseeb (Environmental Protection Authority, EPA, Socotra Branch) are thanked for supporting this research in the frame of the Memorandum of Agreement on Scientific and Technical Cooperation concerning the Socotra Archipelago between EPA and BiK-F. Thanks are also due to Fouad Naseeb and Maren Ziegler (BiK-F, SF) for help with field work and to Florian Wicker (SF) for assistance with lab work. Gavin Gouws (SAIAB) is acknowledged for comments on and improvements of the manuscript.

Supplementary material

12686_2014_278_MOESM1_ESM.pdf (28 kb)
Supplementary material 1 (PDF 27 kb)


  1. Benson G (1999) Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27:573–580PubMedCentralPubMedCrossRefGoogle Scholar
  2. Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform 1:47–50Google Scholar
  3. Gon O, Allen GR (2012) Revision of the Indo-Pacific cardinalfish genus Siphamia (Perciformes: Apogonidae). Zootaxa 3294:1–84Google Scholar
  4. Holm S (1979) A simple sequentially rejective multiple test procedure. Scand J Stat 6:65–70Google Scholar
  5. Rozen S, Skaletsky HJ (1999) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology, 132nd edn. Humana Press, Totowa, NJ, pp 365–386CrossRefGoogle Scholar
  6. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–553CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Tilman J. Alpermann
    • 1
    • 2
    Email author
  • Jörg Plieske
    • 3
  • Ahmad O. Mal
    • 4
  • Ofer Gon
    • 5
  • Michael L. Berumen
    • 6
  1. 1.Ichthyology SectionSenckenberg Research Institute and Natural History Museum FrankfurtFrankfurt am MainGermany
  2. 2.Biodiversity and Climate Research Centre (BiK-F)Frankfurt am MainGermany
  3. 3.TraitGeneticsStadt Seeland OT GaterslebenGermany
  4. 4.Marine Biology Department, Faculty of Marine SciencesKing Abdulaziz UniversityJeddahKingdom of Saudi Arabia
  5. 5.South African Institute for Aquatic BiodiversityGrahamstownSouth Africa
  6. 6.Red Sea Research CenterKing Abdullah University of Science and TechnologyThuwalKingdom of Saudi Arabia

Personalised recommendations