Conservation Genetics Resources

, Volume 6, Issue 4, pp 841–843 | Cite as

Isolation and characterization of polymorphic microsatellites in the perennial herb Euphorbia kansui using paired-end Illumina shotgun sequencing

  • Xiao-Hao Yan
  • Min-Feng Fang
  • Zeng-Qiang Qian
  • Zhan-Lin Liu
  • Ma-Li Wang
  • Cai-Ping Tian
  • Meng-Han Du
  • Yin-Jun Li
  • Zhong-Hu Li
  • Gui-Fang Zhao
Technical Note

Abstract

Euphorbia kansui is a threatened perennial herb endemic to China, and a good knowledge of its population genetics would be essential to the formulation of effective conservation and management strategies. In this study, 12 novel microsatellite markers were developed for E. kansui using Illumina sequencing technology. The number of alleles varied between 2 and 11, with an average of 4.1 alleles per locus. The expected (HE) and observed heterozygosity (HO) ranged from 0.0950 to 0.7875 and from 0.0500 to 0.9500, with an average of 0.4050 and 0.4083, respectively. Following the false discovery rate controlling procedure, significant deviations from Hardy–Weinberg equilibrium was only detected in a single locus (Ek1073). These informative microsatellite markers can be readily used for genetic analyses of this threatened plant.

Keywords

Euphorbia kansui Genetic diversity Illumina Microsatellite markers Polymorphism 

Supplementary material

12686_2014_274_MOESM1_ESM.doc (40 kb)
Supplementary material 1 (DOC 39 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Xiao-Hao Yan
    • 1
  • Min-Feng Fang
    • 1
  • Zeng-Qiang Qian
    • 2
  • Zhan-Lin Liu
    • 1
  • Ma-Li Wang
    • 1
  • Cai-Ping Tian
    • 3
  • Meng-Han Du
    • 1
  • Yin-Jun Li
    • 1
  • Zhong-Hu Li
    • 1
  • Gui-Fang Zhao
    • 1
  1. 1.Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life SciencesNorthwest UniversityXi’anChina
  2. 2.College of Life SciencesShaanxi Normal UniversityXi’anChina
  3. 3.Analysis Experiment CenterGansu Academy of Medical ScienceLanzhouChina

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