Isolation and characterization of polymorphic microsatellites in the perennial herb Euphorbia kansui using paired-end Illumina shotgun sequencing
- 182 Downloads
Euphorbia kansui is a threatened perennial herb endemic to China, and a good knowledge of its population genetics would be essential to the formulation of effective conservation and management strategies. In this study, 12 novel microsatellite markers were developed for E. kansui using Illumina sequencing technology. The number of alleles varied between 2 and 11, with an average of 4.1 alleles per locus. The expected (HE) and observed heterozygosity (HO) ranged from 0.0950 to 0.7875 and from 0.0500 to 0.9500, with an average of 0.4050 and 0.4083, respectively. Following the false discovery rate controlling procedure, significant deviations from Hardy–Weinberg equilibrium was only detected in a single locus (Ek1073). These informative microsatellite markers can be readily used for genetic analyses of this threatened plant.
KeywordsEuphorbia kansui Genetic diversity Illumina Microsatellite markers Polymorphism
- Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar