Isolation and characterization of eight microsatellites from the South American annual fish Austrolebias charrua
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Austrolebias charrua is an endemic annual fish of Uruguay and Brazil. Eight microsatellite markers were developed in order to clarify its population genetic structure. All of them constitute polymorphic loci. The number of observed alleles ranged from 6 to 14, the mean expected heterozygosity from 0.65 to 0.92 and the Shannon-Wiener Diversity Indices from 1.31 to 2.39. This study is the first report of microsatellites in annual killifish and they will be a helpful tool to implement conservation strategies in this endangered species.
KeywordsAustrolebiascharrua Killifish South America Microsatellite loci
We wish to thank Guillermo Ortí for receiving SP in his laboratory on the initial phases of this project, Nicolás Papa for initial technical support. This work was partially supported by scholarships (ANII, Uruguay; to VG and SP), a PDT project (to SP), Project AWARE (to SP) and a CSIC_DT Udelar Project (to GG). GG, SP and VG acknowledge the support of SNI (ANII, Uruguay). The authors are also grateful to IDEA WILD Foundation and the Japanese government for the donation of equipment.
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