Abstract
Cecropia insignis is an ecologically important Neotropical pioneer tree and major vertebrate food source. Although this species is relatively common in faunally intact tropical rainforests, its population dynamics may be negatively impacted by hunting of seed-dispersing animals. To better understand gene flow and regeneration dynamics in C. insignis, we characterized 26 microsatellite markers in a population sampled from Barro Colorado Island, Panama. Eleven loci of ≥3 alleles were tested on 48 individuals, whereas the remaining 15 loci of two alleles were tested on 12 individuals. Allelic richness ranged from 2 to 9 per locus. Observed and expected heterozygosity averaged 0.478 and 0.440 respectively. Polymorphism information content was between 0.141 and 0.757. Only two loci exhibited deviation from Hardy–Weinberg proportions.
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.References
Liu KJ, Muse SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21(9):2128–2129. doi:10.1093/bioinformatics/bti282
Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin JF (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26(3):403–404. doi:10.1093/bioinformatics/btp670
Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28(19):2537–2539. doi:10.1093/bioinformatics/bts460
Terborgh J, Nun ez-Iturri G, Pitman NCA, Valverde FHC, Alvarez P, Swamy V, Pringle EG, Paine CET (2008) Tree recruitment in an empty forest. Ecology 89(6):1757–1768
Wei N, Bemmels JB, Dick CW (in press) The effects of read length, quality and quantity on microsatellite discovery and primer development: from Illumina to PacBio. Mol Ecol Resour. doi:10.1111/1755-0998.12245
Acknowledgments
This work was supported by a CTFS-ForestGEO grant from Smithsonian Tropical Research Institute and Center for Tropical Forest Science.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wei, N., Dick, C.W. Characterization of twenty-six microsatellite markers for the tropical pioneer tree species Cecropia insignis Liebm (Urticaceae). Conservation Genet Resour 6, 987–989 (2014). https://doi.org/10.1007/s12686-014-0265-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-014-0265-x