Characterisation of 9 polymorphic microsatellite markers for the Critically Endangered lemur leaf frog Agalychnis lemur

Abstract

We characterise nine polymorphic microsatellites for the Critically Endangered lemur leaf frog, Agalychnis lemur. We found between 3 and 8 alleles per locus in 48 captive individuals originating from the last two currently known remaining wild populations of these frogs in Costa Rica. We attribute observed deviations from Hardy–Weinberg and linkage equilibrium to inbreeding and population substructure in captivity. The newly designed loci will be used to establish the first genetically-informed amphibian studbook for captive breeding and conservation purposes.

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References

  1. García-Rodríguez A, Chaves G, Benavides-Varela C, Puschendorf R (2012) Where are the survivors? Tracking relictual populations of endangered frogs in Costa Rica. Divers Distrib 18:204–212

    Article  Google Scholar 

  2. Gray AR (2011). Notes on hybridization in leaf frogs of the genus Agalychnis (Anura, Hylidae, Phyllomedusinae). Cornell University and the National Science Institute’s ArXiv:1102.4039v1. http://arxiv.org/pdf/1102.4039v1

  3. Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Res 8:103–106

    Article  Google Scholar 

  4. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning—a laboratory manual, 2nd edn. Cold Spring Habor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  5. Solís F, Ibáñez R, SavageJ, Jaramillo C, Fuenmayor Q, Kubicki B, Pounds A, Chaves G, Jungfer K-H, Lips K (2004) Hylomantis lemur. In: IUCN 2013. IUCN red list of threatened species. Version 2013.2. www.iucnredlist.org

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Acknowledgments

We are grateful to Adam Bland of Manchester Museum, for his help and assistance with the rearing and sampling of captive specimens. Samples in Costa Rica were collected and exported with special permission from Costa Rican authorities: MINAE (collection/export permit: Resolution no: 255-2000-OFAU). We would especially like to thank Snr Javier Guevara for all his support.

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Correspondence to Alex Petchey.

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Petchey, A., Gray, A., Andrén, C. et al. Characterisation of 9 polymorphic microsatellite markers for the Critically Endangered lemur leaf frog Agalychnis lemur . Conservation Genet Resour 6, 971–973 (2014). https://doi.org/10.1007/s12686-014-0261-1

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Keywords

  • Lemur leaf frog
  • Agalychnis lemur
  • Microsatellites
  • Captive breeding
  • Leaf frogs
  • Genetic management