Conservation Genetics Resources

, Volume 6, Issue 4, pp 969–970 | Cite as

Cross amplification of 15 EST-SSR markers in the genus Fraxinus

  • Andi G. Noakes
  • Teodora Best
  • Margaret E. Staton
  • Jennifer Koch
  • Jeanne Romero-Severson
Microsatellite Letters

Abstract

Ash (Fraxinus, Oleaceae) species occur on most continents, within a wide range of forest tree communities. Emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), introduced into the U.S. from Asia in the late twentieth century, has caused widespread mortality, primarily in green ash, Fraxinus pennsylvanica Marsh. (Section: Melioides) and now impacts other North American ash species. The development and successful reintroduction of resistant trees requires genetic tools to evaluate population dynamics and aid in species identification. Here, we report 15 novel EST-SSR markers developed in green ash, most of which amplify and are polymorphic across 18 species of Fraxinus, including six species native to North America. The high average polymorphism information content (0.741) and allelic richness (15.3) revealed in six disparate populations of green ash indicate that these markers also have utility for investigating population dynamics in this species.

Keywords

Fraxinus pennsylvanica EST-SSR Heterologous markers Green ash 

Supplementary material

12686_2014_260_MOESM1_ESM.pdf (130 kb)
Supplementary material 1 (PDF 130 kb)
12686_2014_260_MOESM2_ESM.pdf (66 kb)
Supplementary material 2 (PDF 65 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Andi G. Noakes
    • 1
  • Teodora Best
    • 2
  • Margaret E. Staton
    • 3
  • Jennifer Koch
    • 4
  • Jeanne Romero-Severson
    • 5
  1. 1.Department of Biological SciencesUniversity of Notre DameNotre DameUSA
  2. 2.Department of Ecosystem Science and ManagementPennsylvania State UniversityUniversity ParkUSA
  3. 3.Department of Entomology and Plant PathologyUniversity of TennesseeKnoxvilleUSA
  4. 4.Forestry Sciences LaboratoryUSDA Forest ServiceDelawareUSA
  5. 5.Department of Biological SciencesUniversity of Notre DameNotre DameUSA

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