Conservation Genetics Resources

, Volume 6, Issue 4, pp 969–970 | Cite as

Cross amplification of 15 EST-SSR markers in the genus Fraxinus

  • Andi G. Noakes
  • Teodora Best
  • Margaret E. Staton
  • Jennifer Koch
  • Jeanne Romero-SeversonEmail author
Microsatellite Letters


Ash (Fraxinus, Oleaceae) species occur on most continents, within a wide range of forest tree communities. Emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), introduced into the U.S. from Asia in the late twentieth century, has caused widespread mortality, primarily in green ash, Fraxinus pennsylvanica Marsh. (Section: Melioides) and now impacts other North American ash species. The development and successful reintroduction of resistant trees requires genetic tools to evaluate population dynamics and aid in species identification. Here, we report 15 novel EST-SSR markers developed in green ash, most of which amplify and are polymorphic across 18 species of Fraxinus, including six species native to North America. The high average polymorphism information content (0.741) and allelic richness (15.3) revealed in six disparate populations of green ash indicate that these markers also have utility for investigating population dynamics in this species.


Fraxinus pennsylvanica EST-SSR Heterologous markers Green ash 



This work was funded in part by the National Science Foundation (IOS-1025974). We thank Daniel Borkowski, Arpita Konar, Robert Stanley, and Lauren Fiedler for assistance with lab work and field collections.

Supplementary material

12686_2014_260_MOESM1_ESM.pdf (130 kb)
Supplementary material 1 (PDF 130 kb)
12686_2014_260_MOESM2_ESM.pdf (66 kb)
Supplementary material 2 (PDF 65 kb)


  1. Hoban SM, Borkowski DS, Brosi SL, McCleary TS, Thompson LM, McLachlan JS, Pereira MA, Schlarbaum SE, Romero-Severson J (2010) Range-wide distribution of genetic diversity in the North American tree Juglans cinerea: a product of range shifts, not ecological marginality or recent population decline. Mol Ecol 19:4876–4891PubMedCrossRefGoogle Scholar
  2. Kovacs KF, Haight RG, McCullough DG, Mercader RJ, Siegert NW, Liebhold AM (2010) Cost of potential emerald ash borer damage in U.S. communities, 2009–2019. Ecol Econ 69:569–578CrossRefGoogle Scholar
  3. Liang L, Fei S (2014) Divergence of the potential invasion range of emerald ash borer and its host distribution in North America under climate change. Clim Change 122:735–746CrossRefGoogle Scholar
  4. McKinney LV, Thomsen IM, Kjær ED, Nielsen LR (2012) Genetic resistance to Hymenoscyphus pseudoalbidus limits fungal growth and symptom occurrence in Fraxinus excelsior. For Pathol 42:69–74CrossRefGoogle Scholar
  5. Raymond M, Rousset F (1995) GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Andi G. Noakes
    • 1
  • Teodora Best
    • 2
  • Margaret E. Staton
    • 3
  • Jennifer Koch
    • 4
  • Jeanne Romero-Severson
    • 5
    Email author
  1. 1.Department of Biological SciencesUniversity of Notre DameNotre DameUSA
  2. 2.Department of Ecosystem Science and ManagementPennsylvania State UniversityUniversity ParkUSA
  3. 3.Department of Entomology and Plant PathologyUniversity of TennesseeKnoxvilleUSA
  4. 4.Forestry Sciences LaboratoryUSDA Forest ServiceDelawareUSA
  5. 5.Department of Biological SciencesUniversity of Notre DameNotre DameUSA

Personalised recommendations