Cross amplification of 15 EST-SSR markers in the genus Fraxinus
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Ash (Fraxinus, Oleaceae) species occur on most continents, within a wide range of forest tree communities. Emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), introduced into the U.S. from Asia in the late twentieth century, has caused widespread mortality, primarily in green ash, Fraxinus pennsylvanica Marsh. (Section: Melioides) and now impacts other North American ash species. The development and successful reintroduction of resistant trees requires genetic tools to evaluate population dynamics and aid in species identification. Here, we report 15 novel EST-SSR markers developed in green ash, most of which amplify and are polymorphic across 18 species of Fraxinus, including six species native to North America. The high average polymorphism information content (0.741) and allelic richness (15.3) revealed in six disparate populations of green ash indicate that these markers also have utility for investigating population dynamics in this species.
KeywordsFraxinus pennsylvanica EST-SSR Heterologous markers Green ash
This work was funded in part by the National Science Foundation (IOS-1025974). We thank Daniel Borkowski, Arpita Konar, Robert Stanley, and Lauren Fiedler for assistance with lab work and field collections.
- Hoban SM, Borkowski DS, Brosi SL, McCleary TS, Thompson LM, McLachlan JS, Pereira MA, Schlarbaum SE, Romero-Severson J (2010) Range-wide distribution of genetic diversity in the North American tree Juglans cinerea: a product of range shifts, not ecological marginality or recent population decline. Mol Ecol 19:4876–4891PubMedCrossRefGoogle Scholar
- Raymond M, Rousset F (1995) GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar