Abstract
The brown algal genus Laminaria (Phaeophyceae, Laminariales) is ecologically and economically important. Laminaria ochroleuca is the genus member with the southernmost distribution in the Northern hemisphere, from Southern Great Britain to Morocco. Here we describe the characterization of 15 microsatellite loci that can be used to study genetic diversity at different spatial scales and its dynamics in declining southern populations. These loci identified a number of alleles ranging from 3 to 26 (mean = 9). Expected heterozygosities range from 0.0824 to 0.9401. We also tested cross-amplification of these microsatellite markers in two other species within the genus, L. hyperborea and L. digitata. A total of eight and ten alleles cross-amplified in L. hyperborea and L. digitata, respectively. These 15 variable microsatellite loci are an important molecular tool to conduct population genetics studies in L. ochroleuca and other species within the genus.
References
Ardré F (1970) Contribution à l’étude des algues marines du Portugal. I. La flore. Portugalia Acta Biologica sér. B 10:137–555
Assis J, Tavares D, Tavares JT, Cunha AH, Alberto F, Serrão EA (2009) Findkelp, a GIS-based community participation project to assess Portuguese kelp conservation status. J Coast Res SI 56:1469–1473
Lima FP, Wethey DS (2012) Three decades of high-resolution coastal sea surface temperatures reveal more than warming. Nat Commun 3:704. doi:10.1038/ncomms1713
Raymond M, Rousset F (1995) GENEPOP (version 1.2) population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Santos R (1993) A multivariate study of biotic and abiotic relationships in a subtidal algae stand. Mar Ecol Prog Ser 94:181–190. doi:10.3354/meps094181
Acknowledgments
We thank MJ Martins for laboratory help and M Valero for providing L. hyperborea samples. Financial support was provided by FCT, Portugal, grants MEGIKELP [PTDC/MAR/65461/2006] and EXTANT [EXCL/AAG-GLO/0661/2012].
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Coelho, N.C., Serrão, E.A. & Alberto, F. Characterization of fifteen microsatellite markers for the kelp Laminaria ochroleuca and cross species amplification within the genus. Conservation Genet Resour 6, 949–950 (2014). https://doi.org/10.1007/s12686-014-0249-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-014-0249-x