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Conservation Genetics Resources

, Volume 6, Issue 4, pp 949–950 | Cite as

Characterization of fifteen microsatellite markers for the kelp Laminaria ochroleuca and cross species amplification within the genus

  • N. C. Coelho
  • E. A. Serrão
  • F. AlbertoEmail author
Microsatellite Letters

Abstract

The brown algal genus Laminaria (Phaeophyceae, Laminariales) is ecologically and economically important. Laminaria ochroleuca is the genus member with the southernmost distribution in the Northern hemisphere, from Southern Great Britain to Morocco. Here we describe the characterization of 15 microsatellite loci that can be used to study genetic diversity at different spatial scales and its dynamics in declining southern populations. These loci identified a number of alleles ranging from 3 to 26 (mean = 9). Expected heterozygosities range from 0.0824 to 0.9401. We also tested cross-amplification of these microsatellite markers in two other species within the genus, L. hyperborea and L. digitata. A total of eight and ten alleles cross-amplified in L. hyperborea and L. digitata, respectively. These 15 variable microsatellite loci are an important molecular tool to conduct population genetics studies in L. ochroleuca and other species within the genus.

Keywords

Laminaria ochroleuca Microsatellites Diversity Cross-amplification Population genetics 

Notes

Acknowledgments

We thank MJ Martins for laboratory help and M Valero for providing L. hyperborea samples. Financial support was provided by FCT, Portugal, grants MEGIKELP [PTDC/MAR/65461/2006] and EXTANT [EXCL/AAG-GLO/0661/2012].

Supplementary material

12686_2014_249_MOESM1_ESM.doc (52 kb)
Supplementary material 1 (DOC 51 kb)
12686_2014_249_MOESM2_ESM.doc (46 kb)
Supplementary material 2 (DOC 46 kb)

References

  1. Ardré F (1970) Contribution à l’étude des algues marines du Portugal. I. La flore. Portugalia Acta Biologica sér. B 10:137–555Google Scholar
  2. Assis J, Tavares D, Tavares JT, Cunha AH, Alberto F, Serrão EA (2009) Findkelp, a GIS-based community participation project to assess Portuguese kelp conservation status. J Coast Res SI 56:1469–1473Google Scholar
  3. Lima FP, Wethey DS (2012) Three decades of high-resolution coastal sea surface temperatures reveal more than warming. Nat Commun 3:704. doi: 10.1038/ncomms1713 PubMedCrossRefGoogle Scholar
  4. Raymond M, Rousset F (1995) GENEPOP (version 1.2) population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
  5. Santos R (1993) A multivariate study of biotic and abiotic relationships in a subtidal algae stand. Mar Ecol Prog Ser 94:181–190. doi: 10.3354/meps094181 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.CCMAR, CIMAR-Laboratório AssociadoUniversity of AlgarveFaroPortugal
  2. 2.Department of Biological SciencesUniversity of Wisconsin-MilwaukeeMilwaukeeUSA

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