Characterization of fifteen microsatellite markers for the kelp Laminaria ochroleuca and cross species amplification within the genus
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The brown algal genus Laminaria (Phaeophyceae, Laminariales) is ecologically and economically important. Laminaria ochroleuca is the genus member with the southernmost distribution in the Northern hemisphere, from Southern Great Britain to Morocco. Here we describe the characterization of 15 microsatellite loci that can be used to study genetic diversity at different spatial scales and its dynamics in declining southern populations. These loci identified a number of alleles ranging from 3 to 26 (mean = 9). Expected heterozygosities range from 0.0824 to 0.9401. We also tested cross-amplification of these microsatellite markers in two other species within the genus, L. hyperborea and L. digitata. A total of eight and ten alleles cross-amplified in L. hyperborea and L. digitata, respectively. These 15 variable microsatellite loci are an important molecular tool to conduct population genetics studies in L. ochroleuca and other species within the genus.
KeywordsLaminaria ochroleuca Microsatellites Diversity Cross-amplification Population genetics
We thank MJ Martins for laboratory help and M Valero for providing L. hyperborea samples. Financial support was provided by FCT, Portugal, grants MEGIKELP [PTDC/MAR/65461/2006] and EXTANT [EXCL/AAG-GLO/0661/2012].
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