Skip to main content

Advertisement

Springer Nature Link
Log in

Characterization of 57 microsatellite loci for Rapana venosa using genomic next generation sequencing

  • Microsatellite Letters
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

A total of 57 polymorphic microsatellite loci were developed for Rapana venosa through 454 sequencing. The 57 loci were characterized in 30 wild individuals. The number of alleles per locus varied from 2 to 14. The observed and expected heterozygosities ranged from 0.100 to 1.000 and 0.127 to 0.919, respectively. Thirty-two loci were in Hardy–Weinberg equilibrium. The microsatellite loci described in this study will facilitate investigation of conservation genetics of this species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  • Chandler EA, McDowell JR, Graves JE (2008) Genetically monomorphic invasive populations of the rapa whelk, Rapana venosa. Mol Ecol 17:4079–4091

    Article  CAS  PubMed  Google Scholar 

  • Davey JW, Hohenlohe PA, Etter PD, Boone JQ, Catchen JM, Blaxter ML (2011) Genome-wide genetic marker discovery and genotyping using next-generation sequencing. Nat Rev Genet 12:499–510

    Article  CAS  PubMed  Google Scholar 

  • Dieringer D, Schlötterer C (2003) Microsatellite analyser (MSA): a platform independent analysis tool for large microsatellite data sets. Mol Ecol Notes 3:167–169

    Article  CAS  Google Scholar 

  • Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94

    Article  CAS  PubMed  Google Scholar 

  • Yang J, Li Q, Kong L, Zheng X, Wang R (2008) Genetic structure of the veined rapa whelk (Rapana venosa) populations along the coast of China. Biochem Genet 46:539–548

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The study was supported by the grants from Scientific and Technical Supporting Program (2011BAD13B01), National Natural Science Foundation of China (31201998), and Promotive Research Fund for Young and Middle-Aged Scientists of Shandong Province (BS2012HZ020).

Author information

Authors and Affiliations

  1. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Xiaoqi Sun, Hong Yu, Ruihai Yu & Qi Li

Authors
  1. Xiaoqi Sun
    View author publications

    Search author on:PubMed Google Scholar

  2. Hong Yu
    View author publications

    Search author on:PubMed Google Scholar

  3. Ruihai Yu
    View author publications

    Search author on:PubMed Google Scholar

  4. Qi Li
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Hong Yu.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 89 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sun, X., Yu, H., Yu, R. et al. Characterization of 57 microsatellite loci for Rapana venosa using genomic next generation sequencing. Conservation Genet Resour 6, 941–945 (2014). https://doi.org/10.1007/s12686-014-0247-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-014-0247-z

Keywords