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Development of polymorphic microsatellite loci isolated from the Ancherythoculter nigrocauda

  • Microsatellite Letters
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Abstract

Twelve polymorphic microsatellite markers from Ancherythoculter nigrocauda were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. These loci were characterized by genotyping 48 individuals. The observed number of alleles per locus ranged from 4 to 14 with an average of 9.4. The expected and observed heterozygosities ranged from 0.185 to 0.907 and from 0.083 to 0.952, respectively. Among these polymorphic microsatellite loci, four of the loci (HWB01, HWB04, HWB12 and HWB16) significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction (P < 0.05). All of the microsatellite loci were in linkage equilibrium. These microsatellite markers would be useful for investigating the genetic diversity and population structure of A. nigrocauda.

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Acknowledgments

This research is funded by Wuhan Chenguang Project for Youth Scholar (No. 201372304010832).

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Authors and Affiliations

  1. Wuhan Aquaculture Science Research Institute, Wuhan, 430207, China

    Yanhong Sun, Guiying Wang, Dongmei Zhu, Jian Chen, Pei Li & Qing Li

  2. Wuhan Academy of Agricultural Science Technology, Wuhan, 430071, China

    Yanhong Sun

  3. Wuhan Xianfeng Aquaculture Technology Co. Ltd, Wuhan, 430207, China

    Yanhong Sun, Guiying Wang, Dongmei Zhu, Jian Chen, Pei Li & Qing Li

Authors
  1. Yanhong Sun
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  2. Guiying Wang
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  3. Dongmei Zhu
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  4. Jian Chen
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  5. Pei Li
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  6. Qing Li
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Corresponding author

Correspondence to Qing Li.

Appendix: Nucleotide sequences of 16 microsatellites for Ancherythoculter nigrocauda

Appendix: Nucleotide sequences of 16 microsatellites for Ancherythoculter nigrocauda

>HWB01

TCAGTGAAAGTGTTGATCCGTGCTGCAAAACTATATTGCAAGAGAATGGCATTGAAGTGACGGAGAAACCGCAGATGACTAAAGAAGAGTTGATTGCAGAGATTCAGGTAAGTGATTCTTCTGACTCAAGTTCTGCACCCGAATATCAAGTGCCCTGATTTGAATCGCCGCCAAGCTAATGGCGTCCTCCAATGCAGCAACAGTAAAAATGATAAACTATTAGCATGTGGATGTGTGCCAGTTGGATGTGCTAAAGTTTGCGTGTGACTGTGTGTGTGTCACCCCTCCTCCTCCTCTTCAAGAGTCTGATGCAGCAGCAGCCTGTATCTGCACCATATGAATCATTTCAATCGATTCACAACGCATGGTTTTATTAGACCTCAAACAGTAAATATGATTTTTTCCCCCCAGTACAGTATTCT

>HWB02

GACGGTCAGTTTGTCTGGATTGCTGTCTGTGGCCAAGTGTTTTTGTATAAACAATACTGATGTTCTCACATACTTTCCAGAAAGGCGTTTTGGAACCACATACAGTCAACTGTGATTGTGAGATCTGCCAAGAACTGGATCAATACCTAGTAAGACATAATCGCTTGCAATTACACAGTCATGTCCAAACATCAGCATGACCATCGAAATTCATCACAAAAATCTGAAATTATTTCTAAAATGTGCTTTGTCATTTCTTTATGAACTCTTGTCTCAGCAGAATGATGACGTGTGTTTCGTGTGTGAGTCTGAGGGAGATCTTGTGTGTTGTGACGAGTGTCCACGAGCTTTTCATTCACACTGTCATCTACCAGCTGCAGACGGAGACTCACTTGGGTGAGAGAGAGAGAAATCATGCTGGTTTAGACAAACTAAACAGTTGACTTTGTGTAATATTGTGTGTTTTGTCTGTACAGAAGCCAGTGGAGCTGCACATTCTGTGTGATGAAGAATATGAAAGATTCCAGTCAAAAGACCCAACAGGAAGTTTTGAGCAGTCCAGTCTCTCAGTACACACTGGTTTGTGCAAAACTTTTTGTCCTTACAATGGAAGTCAATGGGCTCCTAAATAACATTTATTTTACCATATAGATGAAAAATTTCAACTTTTTGTGTGAACTAACACTTACAGGTATGTTTGTTTCTTTTTTCTCTTTCAGCACTGCCAGTGTTTGCTGTTACACTTGCTACATGAGAACATGACTGACCCCTGCACCAATGTAAGTATCATCTTTAGCATCTTCATCTGTCTGTT

>HWB03

GGGAGTCTGAATACTTTCCGTATCCACTGTATTTATGCATATCTCCCTGCTGAAGATCCTGAAGCCACGTAGACCTAAATATGAGGACACGGTTCACACACGTACACACACAGACAGATGTGATGGATATAGCAAGATGGCTGTATCAGCGAAACAGGTTGTAATAACATCTCACACTGTACCTGATATAGATCAAAATGTCCCTGTGTATCTCTCTCTCTCTCTCTCTCTCTCTCTCTATCTTTGTCTCTGATTTCAGCTGGGTGAGCTGGATGGGGTGGAGAAACCACAGCCGCACTGGTTTCACACGCTGCAGGTGCGACGGCTCAGAGTTCAGAGGGGCCGCAGTAACAGCGATCCTATGGGAGGAAAGAGCTTCCAGCAAGAGTTCCAGTGGGTGAGGGACACACTAATACAACTCAGACATACACCAATACACAACATGTGATT

>HWB04

GAATTACTTCCAATCCTAGAGAATGTCTGAGAAAGGTAAGAACTTTAGGAAGTGTGCTGATCCGTGTCCGAGATTCTTGACACCGGAATACACGCACATCCTATGTGTTCATATGCCTTACAAACGTGCAAGAGAGTGTGTGTATCTGTGTCCCCATGTTTGACACTTGATATACACATTTTTCTAGGTGTTTTCTGTCTGGAGAGGAGCGCGCATAGACGGTGTTTGAGGGCGCTGTCTGTGTGTTTGTTTGTTGTTTACTGTGAGCGTCACCCTATCGGGATGCTCCGTTCTCGTTTGACACTCTTCTCAAGGGAAGGGGTGTCTACATCTGTGCCTGGTGATTCTGGCCGGGTTATGCCGAGGCGAAACCGTGCCTACAATCATAGGGCTCGCAGATGAGCTTGTTGGGAGTTGTGAGGGAGTTGTATTCTCTCTCTCAACTTCCTTGGGGTTGGCGAGAACGAGTGGATGACGATGACGTTCGTTTTTGACGTCATCGCGTCTGGTGAGCGCTCCTCTGGCTTGTGCATGCAAGCCGCTGTGTGTTTGGGACGCGCCTCTCGGCGGGCTGCAGCTGCCGTGAAAGCACGTTAACTCTTGACAAGGCACACCTGTGAATTGAAAACCATTCCAAGTGACCACCTCGTGAATCTGATGAGAGAATGCCACGCTGTCATCAAAGCTAAATGTAGCTACTTTGGACAATCTAAAATATAAAACACATCCTGGTTGTT

>HWB05

TAAAGGGGTACAGATAGGTAACGGCAGCGAGCAGATACTGTTCCAGAAGCGTCTCCATCCTTCAGTCACCTCGGTCTACACTTTCCGCCCACAGAGACTCGCACCAGCGTGTCATTTTCAGCACACTGGCAGAGTGAGAAAGACTGTGTGTCTGTGTGCACATATGTGTGTGAGCCTGTCATTTGGACCCCATGGGACCCAGTAAGTTTACAGCAAGTGATTTACTCAAACTCAGTGCAGCATGTTGTTTGCCAGATGGGCACCAAACTGGCAAGTGTGTGTGTACATATCAAGGCTGGTGCTGGTGAGGGTTTCGTGCGGTGGGTGAACGTTCTGGTCTTGCTGCTCAGACTGCCAGCCTCTTGGGGTGGGCAGATGAGAGGGATCCATAAGGCAGAACTCCTCCAAAATGTTGTTTTCTGCATCAGGTCTTTTCCTGTCCTGTCACAGATACAGCTTTCTCCCCCAGTCTCTGAATAAAACACAAGTACACACTTTTGATAATCACAGCATAGT

>HWB06

TAAAATTAGTGTAAGTGGTGATTGATAGTGGATAATAATATATGTATAATGTACTGACTTATGTAAAATGTACTCACATTTTAGACACAATATTGTTTCTTTACTAAATGATTCAGCATTTTGAATGAATCAGCTGAGTCAAAGATTCAATGACCCATTCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATCTCTGCGTGTGTGTAGTGAGTGTGTGTGTCTGTTTTGTACTCTTGTATCATCCCAGTAAAATGCATGTTTGAGATGTTTTAACTAAAAGCAACTTGCACTGACATATCT

>HWB07

TAAATACACACTCAAACACCTATTCACAATGGACAATGGTTTCGTCAGCTTCCGGTTGCGCTGCGCCAGCGCGACCAGGCCATGACGTCATAAGCAGCTGCCGCTCGATCGTTCGGTTTGGCCAGCGCAGCGTTTCCCACCAGAAGACTGACTACGCCCACATCGACAAACAGCACAGAGTCGAAACAAGATGACAAGTGCAGTGTAGGTGTATGAAATGACCGGGAAATGGATATGGAATAGATGACAGGAGGAGAGTGGAGAAATGGGAGAATGTCCATGTGCTATCGTGATCGTGTGTGCACCCGCTACTACGATAGCGGCAACGGCTGGGAGAAACGATACAGAGAATGAATGAGGTAATTAGATGGGTGTGTGTGTGTGTGAGCGCGTGCGCGTGCATGCCAGTGCTAATCTAAAATGATGCTTCCCCAGTGGTGGCAGCTAATGTGACTTTATCACATCATTTTCCCCCTCTCCAAGCCACGTACTGGCCGGAAACCTGGACAGGTAGTTGGGTACCACATTGGTGCCGTATCT

>HWB08

GATACATGGTTGTGGAGTGCAATGCTGTACCACAACTCCTCTACCATTGAACGGCATCTTCACACACGCTCACGTTGACAAACTCACACAAACACACATCTGCTGATGTGAATCATTAGAGCCAGTGCTGAGGCTGGTCTTTGAGGGTGCTCTGCTCAAGCTAGCTGGAAAGCCTAATGAGCTAGCAAATAACTGCAGAGACCAAGGAAACACACAAAAGCTGCACACACATTCGTGCTCGCGGCCGTGCGTGGTGGTCTGCGGGCCTCATGCGGCGTCTGCGAGAATCAGAAGAGACAGAGGTGTTTATCTGCTCTTCCCTCTGCTGCACATGGCCAAATCAAACGAGAGGAGAAAGTTTCCTATTCTATAAAAGCGCTTTAGATCAATTGCGAGCATCCAGCCTACATCAAACCTGTTTTACACAGAGAGAGAGAGAAAGCAAGAAAGAGGAGGAGGGAACAAAAAGTACAGGAGGAGACTACAATTTGGCAGCACGAGAAATGCTGCTTTCAATCTGCCATTGGGCGGCACAGGCCTAAATACGAGTGACAGAGAAATAAATGCAAAGTTCATCCCAACAGAGCAATGCTGAAAAGTATTTGATCAAATGGAATTGAGGGAGAAGATAGGAGGTTTTACTTTTTATTGTGTTTGTTTGTTTCTTTTCAATGAGTAGATATCAGCACTAAATGTTATAATATT

>HWB09

AAAGTGTTACCAATATTGGTTCAGTACTGATACTAATACTTCTAATTAGGGGCAGTTGTGGCCCAATGGTTTTGTAACCCAAAGGTCGCAGGTTCGATTCTCAACACCAGCAGGAAACGACCTTGAGCAAGGCACCGAGCCCCTAATCGCTCCGCGGGCGCTGCAATGAATGGCCTGCCTCTGGGTGTGTGTGTCACGGTTTGCAGTGTGTGTGTGTGTGTGTGTGCATGTGCAGATTGTGCACTAACTTGGATGGGTTAATACATTATGAATAATCATACATTGATTACTTATGTAGTAGTTTGTGTGTGTGTGTGTGTGTGTGTGTGCAACATTGCTTGGCTCTTTCTGTAGGTTTGCTGTTTGTCTGTGTGTTATGTACAGGGTCATGCACAACATTTGCCT

>HWB10

ACTGAGCCATATTGAGCAGAAGAGACTTCTTACGGTAGTGAAATATAATGCAGGCTTTAGATAGATGTTATATAAGGAAACTGGAAGTGTGTGTGTGTTTATGTGAGCATTTGTTGCAATTGTGTTGTGTTGATATATGTCAAAACGTCGGTATGTGTTTGAAGCAAGTGTTTATTTGGTTAGGTGCGAGTTAGCCAACATGTGTGTGTTTCGGATGTGTTTCTGAGCTCTTGTGTGTGTGTGTGTGTATAAAGTTGATGAGAGAGACCTTTCCCCCCTCGCCTCAGTCTCACTCTTCCACCTACAGCCGTGTTTTTTCAGGCTTCGGACCAGAAGTAAACACTTTGTCCCGGCTTTTGTTGTACGTGTGTGTGTATGCGTTTGACTCTTGGCGCTCGGTGGTGAAATAGGAAAGCCCTTCCTTCACAT

>HWB11

CAGGATGTTTTTTGAACACAACGTTGGGTCAGCAGGACATCAGGTAAGTAAACTGTGCAATGATACTTTAGCAGAGAGAACGAGGATAATGATATCAGTCTTTATCCAGACTGATATCATTATCATATCTGAATCATTATCAGGACTAGGATATCGTGGATGACAGATGATTGGAGGAAGCACACACACACACTTGCAGTTTGGTAGAAACGCACAGAGACAATGGAGGAATAGGATGAGGAGAACACACTGTAGAATCACAAGAGATAAGTACAGAATCGACAATGGAGAACTTGCTTGGAGAATTCGTAGGAGAAACTGGAAATGAGTCCTTTACGTGTGAACGAAACCGGACACTGGAGTGAGGTAAGGTGTGTGCTTTTGAAGCCAGCGTGATGACTGTGCTGATGAGTGGCATGTGCTTGTGATCAGTACTCTGGTGAGGAAGTGCGCTGTGATTGGCTGATGTGGGAGCCTGGCTGATCTGTGACACCCCTGTCGAGCGCATTGCTGGAAGATGTGTGTTGAACATCCACTTTGCATT

>HWB12

AAATAGGCCTAACTGTTTTCTCGGGATCAAAGCTTGCACAAATCGAACAACATAAACAAGCGGTGACACCCGACTTGTGAGTAACTGATCTCTTGCACTCCCATCTGTGTCTGCATGAGGGTCACTCCCATGGCAGACATCTCTCGCCTTTTAGAGTTGCTCCCTGTGACCTGTAACCTGCAAATTATCAATCCCTTTTCCCACTCTTTATCAAAGCCTGCTTGCTCTCATCTTAGTGGCAAAAGTTGCAAAGTTGTGTCCTGCTGAGCGGTGTTCAGGTTGTGAGGTAGCTGTGGCAGAAAATGGTATCAGGTGTGTGTGTGTGTGTCTGTGTGTATGGAGGTGTCTGTGGAGCAGTCTACACCTGCTGTCTCTCTTCAGTGCCCATTGGGGCTGGATTCCCAGAATGCCTTTCAGGCAGCTGTTGACAGCCAGCACTGTGCCCTGGCCCCCCAAACGGCTCTGGGGATCAGCTGCT

>HWB13

AAAATATAGCAATACCAAAATACAAACAATGTAGAATAGCTTGAATACAGCGTGCGTCTCCCTCAGACTGTAAATAAAGCTCAGGCTCACTACATAACACGTCATCAGCGTCACACTCCGGAGCAGAAGGGGGCGGTAATGCACCAATAAGCTGGATGCCAACCGCCGTAAAACAGGAAAGAAGAAGAAGAAGAAGCGTGAACGCGAATTGACAACAGACCCGGAAGAGAAGACAATGCTGAATAAAATCGTAGTTTTTGTTATTTTTGGACCAAAATGTATTTTCGATGCTTCAACAAATTCTAACTAACCCACTGATGTCACATGGACTACTTTGATGATGTTTGTATTACCTTTCTGGACATGGACAGTATACCGTACATACATTTTCAATGGAGGGACAGAAAGCTCTCGGACTAAATCTAAAATATCT

>HWB14

ATGCCCTTCCCTCGCTAACTTCCCTCGGTCTCGTTGACTCGGATGTACGTCATTGCTTACGTTGCACGAGTGCCCACTTCTGGCGGAACCTTTGCAATGGGCTGAACTGGAACGTCCTAAGCCCTTGATCACTTGGAATCCGCAATGGAGCTGATTTATTATTTATTTTTTCCTCTTACAAATTTGTTTAATGTCAACTACTTATGTGTGCGCGTCTACGTGTATGGCTGCATGTATAGTGTATGTGTGTGTGTGTGTGTTGAGTGTGATCAGTGGAAAGTGAATGAATAAATAATAGGGGCGGCAAACGGAGGCAGCTGCTCTCTGTTGGCTTCTGAAGTGGCGCGGGGTGAGGTGAGACAGGCGGCGGTGGAGAGTGCCGTTTATTCACAACCAGGAGTCTGTTTACCGGAACAAACCAGTCGCCACAGCTACT

>HWB15

AACGAACCCTGGTGCAGTTGCTCGGTTAGTGCGGTTCATTTGAACATATGTGAACGCTGCCATCCGAACCCTGGTGCACACCAAACAAACTCTGGTGATATATGAACGCAACACGGACCAAAGACATGTAACCGAACCAAAAACAGGAAGACGAGACCCTAAAAAGGAGAGAATCCTCACGCATGTCGGTTTTTCTTGTCATAGGCGCGAGTTTGCTCATCATCACAGGCATCAGACGCGCGTCTCCACGCAGCAGATCGTTTGTGTGTGTGACGGATGGATTCCCGCCGGTGTTTTGACTACTTTACACATTTTATAAGCTCTTCACGAGTTCCCAACTGGCCAAAATACCATCACATGCAGGCTACGCACCGCTACACACACACAACGAGCACATTTACCTCAGCAAACAGCATTGTTTTGGATGTTCGGTAAGTTCCGTCTCAAAATAGGCAATACATCATAAAATCCGACCAATCACGTTGTGAATGTATCCCTATGCCT

>HWB16

CAAAGCTGGCGGACAGCAGTGCCCCGATTCTGTTGGAGGTCCCTCTCTCCGGACCACTAGCTCATCCAGGCAGTAAAAGAAGGCTGACAGGGATACAGATGGTCTAGTATACATAGTATAAACAACAACTGGCTTATGAAGGTGTCGTAAATGCCAGGAGCAGCTGGCTACCAGAGCACAAAGTGGAACGAAAAGAAAAAGAGATATGATAAGATAGAGAGAAAGAACAGGGGAAATTGTTTACATGTGTGCTTGGGTGTGGGCACATATGGGTGTGTAGGGTTTGTGTGGGTGTGCAGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTTCACCCAGGGACCTGGGGAGAGAATACATCTGGTAGGACAGCTGTTAGCGGTGTACAAGAGTATACTGATGGTGGTGAGGAATGATAAATCACAGACTTTGCAGCATTGCAGACAGTGCACCAACATACCTGTGAGTGCAGGTACTCAGGCACTGTCAGAAATGCTAAATAATCAATGACCCTATCCAAACAACACAGCTACAACTGTCTAAATGTGTCCGACTTGACAGTGACAACTGCGTAAAGCAAATTTGTCTCGAAATGAACACATT

Note: The repeat sequences for each microsatellite are underlined.

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Sun, Y., Wang, G., Zhu, D. et al. Development of polymorphic microsatellite loci isolated from the Ancherythoculter nigrocauda . Conservation Genet Resour 6, 919–923 (2014). https://doi.org/10.1007/s12686-014-0241-5

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