Characterization of 10 microsatellite markers for the southern torrent salamander (Rhyacotriton variegatus)
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Rhyacotriton variegatus is an endemic salamander species with specific habitat requirements and a restricted geographic range in the northwestern United States. Although not currently listed at the state or federal level, R. variegatus is vulnerable to habitat loss from timber harvest. We isolated and characterized 10 polymorphic microsatellite loci among 367 individuals from across the species’ range. The number of alleles per locus ranged from 7 to 16, observed heterozygosity ranged from 0.16 to 0.58, and expected heterozygosity ranged from 0.53 to 0.87. These new loci will assist in examining the genetic diversity and structure of R. variegatus and potentially related species.
KeywordsAmphibian Rhyacotriton variegatus Microsatellite PCR primers Genetic diversity
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