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A complete mitochondrial genome sequence for the Australian turtle, Chelodina longicollis, obtained using 454-pyrosequencing

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Abstract

The mitochondrial genome of the Australia eastern long-necked turtle Chelodina longicollis is 16,437 bp, containing 37 genes, with overall gene organisation and nucleotide composition typical of mitogenomes of other vertebrates. We identified a presumptive light strand replication initiation site (OL) in the WANCY region, a feature thought to be absent from the side-necked turtles (Pleurodira). The tRNAser2 of C. longicollis lacks a D-arm. This is the fifth mitogenome published for the Pleurodira, the third for the Chelidae and the first turtle mitogenome generated using next-generation sequencing. It will assist the development of primers and strategies for examining mitochondrial variation across the side-necked turtles.

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Acknowledgments

We than Denis O’Meally for help in bioinformatics and Kate Hodges, Clare Holleley, Denis O’Meally, Todd Castoe and Peter Unmack for constructive comments on an early draft of this manuscript.

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Authors and Affiliations

  1. Wildlife Genetics Laboratory, Institute for Applied Ecology, University of Canberra, Canberra, ACT, 2601, Australia

    Xiuwen Zhang & Arthur Georges

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  1. Xiuwen Zhang
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  2. Arthur Georges
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Correspondence to Arthur Georges.

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Zhang, X., Georges, A. A complete mitochondrial genome sequence for the Australian turtle, Chelodina longicollis, obtained using 454-pyrosequencing. Conservation Genet Resour 6, 555–557 (2014). https://doi.org/10.1007/s12686-014-0202-z

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  • DOI: https://doi.org/10.1007/s12686-014-0202-z

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