Conservation Genetics Resources

, Volume 6, Issue 3, pp 551–553 | Cite as

Primers for detection of Macquarie perch from environmental and trace DNA samples

  • Anna J. MacDonaldEmail author
  • Matthew J. Young
  • Mark Lintermans
  • Stephen D. Sarre
Technical Note


Predation by invasive salmonids threatens the conservation of the endangered Australian freshwater fish Macquarie perch (Macquaria australasica). Predation is difficult to detect visually because larval fish are rapidly digested. Here, we present PCR primers that will enable the detection of M. australasica from trace samples, removing the need to detect undigested larval fish to confirm predation. We tested these primers on DNA from all fish species present in an upstream section of the Cotter River, a key locality for the species. We demonstrate the primers to be species-specific, amplifying DNA from M. australasica only.


DNA detection Environmental DNA Species detection Species-specific primers 



Thanks to Ben Broadhurst, Rhian Clear and Sally Hatton. Fish were collected under ACT licences LT2011505 and LT2010411 and animal ethics approval CEAE 10-07. This work was funded by ACTEW Corporation and the Bulk Water Alliance.


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Anna J. MacDonald
    • 1
    Email author
  • Matthew J. Young
    • 1
  • Mark Lintermans
    • 1
  • Stephen D. Sarre
    • 1
  1. 1.Institute for Applied EcologyUniversity of CanberraCanberraAustralia

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