Primers for detection of Macquarie perch from environmental and trace DNA samples
- 227 Downloads
Predation by invasive salmonids threatens the conservation of the endangered Australian freshwater fish Macquarie perch (Macquaria australasica). Predation is difficult to detect visually because larval fish are rapidly digested. Here, we present PCR primers that will enable the detection of M. australasica from trace samples, removing the need to detect undigested larval fish to confirm predation. We tested these primers on DNA from all fish species present in an upstream section of the Cotter River, a key locality for the species. We demonstrate the primers to be species-specific, amplifying DNA from M. australasica only.
KeywordsDNA detection Environmental DNA Species detection Species-specific primers
Thanks to Ben Broadhurst, Rhian Clear and Sally Hatton. Fish were collected under ACT licences LT2011505 and LT2010411 and animal ethics approval CEAE 10-07. This work was funded by ACTEW Corporation and the Bulk Water Alliance.
- Lintermans M (2002) Fish in the upper Murrumbidgee catchment: a review of current knowledge. Environment ACT, CanberraGoogle Scholar
- Lintermans M (2007) Fishes of the Murray-Darling Basin: an introductory guide. Murray-Darling Basin Authority, CanberraGoogle Scholar
- Wager R (1996) Macquaria australasica. In: IUCN 2013. IUCN Red list threat. Species. Version 2013.1. <www.iucnredlist.org>