Skip to main content
SpringerLink
Log in

Development and characterization of thirteen polymorphic microsatellite loci in the Cynoglossus semilaevis

  • Microsatellite Letters
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Thirteen polymorphic microsatellite loci have been isolated and characterized from Cynoglossus semilaevis for the purpose of protecting its wild resources. They had between three and nine alleles. Observed and expected heterozygosities varied from 0.57 to 0.93, and from 0.56 to 0.82, respectively. All the loci conformed to Hardy–Weinberg equilibrium after Bonferroni correction. No significant linkage disequilibrium was detected among the loci (P > 0.004). They have provided sufficient level of genetic diversity to investigate the fine-scale population structure and species conservation and management in the C. semilaevis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Benson G (1999) Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27:573–580

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Li ZB, Lei GG, Chang JB, Zhao BL, Chen J, Zhang GL, Wang ZL (2011) Comparison of genetic diversity of wild and cultivated populations of Cynoglossus semilaevis. Oceanol Limnol Sin 42:414–418

    CAS  Google Scholar 

  • Liu YG, Chen SL, Li BF (2005) Assessing the genetic structure of three Japanese flounder (Paralichthys olivaceus) stocks by microsatellite markers. Aquaculture 243:103–111

    Article  CAS  Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225

    Article  Google Scholar 

  • Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by a grant from Yantai University (SM13B22).

Author information

Authors and Affiliations

  1. College of Life Sciences, Yantai University, 32 Qingquan Road, Yantai, 264005, China

    Yun-Guo Liu & Ling-Xiao Liu

  2. Linyi Academy of Agricultural Sciences, Linyi, 276012, China

    Ling-Xiao Liu

  3. Gout Laboratory, The Affiliated Hospital of Qingdao Medical College, Qingdao University, Qingdao, 266003, China

    Shi-Chao Xing

Authors
  1. Yun-Guo Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ling-Xiao Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shi-Chao Xing
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Yun-Guo Liu or Shi-Chao Xing.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, YG., Liu, LX. & Xing, SC. Development and characterization of thirteen polymorphic microsatellite loci in the Cynoglossus semilaevis . Conservation Genet Resour 6, 683–684 (2014). https://doi.org/10.1007/s12686-014-0181-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-014-0181-0

Keywords

Search

Navigation