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Development of 39 polymorphic microsatellite markers for the jumbo squid Dosidicus gigas using Illumina paired-end sequencing

  • Microsatellite Letters
  • Published:
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Abstract

Dosidicus gigas is an abundant squid species supporting one of the most important fisheries in the Eastern Pacific Ocean. Assessment and management of the fishery requires reliable information on the population’s genetic structure. In this study, we isolated and characterized 39 novel polymorphic microsatellite loci for D. gigas using Illumina paired-end sequencing. Loci were screened in 20 individuals from the Eastern Pacific Ocean. The number of alleles per locus ranged from 4 to 26 (mean = 14.72). The observed heterozygosity ranged from 0.150 to 0.950 (mean = 0.604), and the expected heterozygosity ranged from 0.487 to 0.972 (mean = 0.872). The polymorphic information content ranged from 0.440 to 0.945, with a mean of 0.836. These microsatellite loci are expected to be useful for further studies of genetic diversity and genetic structure of D. gigas.

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Acknowledgments

We thank J. J. Fu for assistance with data analysis. We are grateful to W. Yu and C. J. Wang for tissue collection. This work was funded by the National Science Foundation of China (NSFC41276156), State 863 projects (2012AA092303), and the Shanghai Leading Academic Discipline Project (Fisheries Discipline, Code A).

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Authors and Affiliations

  1. College of Marine Sciences, Shanghai Ocean University, Hucheng Ring Road 999, Lingang New City, Shanghai, 201306, China

    Lianwei Liu, Xinjun Chen, Qianghua Xu, Siquan Tian & Bilin Liu

  2. The Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources, Ministry of Education, Hucheng Ring Road 999, Shanghai, 201306, China

    Xinjun Chen, Qianghua Xu, Siquan Tian & Bilin Liu

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  1. Lianwei Liu
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  2. Xinjun Chen
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  3. Qianghua Xu
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  4. Siquan Tian
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  5. Bilin Liu
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Corresponding author

Correspondence to Xinjun Chen.

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Liu, L., Chen, X., Xu, Q. et al. Development of 39 polymorphic microsatellite markers for the jumbo squid Dosidicus gigas using Illumina paired-end sequencing. Conservation Genet Resour 6, 673–676 (2014). https://doi.org/10.1007/s12686-014-0179-7

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  • DOI: https://doi.org/10.1007/s12686-014-0179-7

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