Development of 24 tetra-nucleotide microsatellite markers in Cortes Geoduck Panopea globosa by next-generation sequencing
Panopea globosa is a marine bivalve mollusk important for small-scale fisheries from Northwest Mexico. Twenty-four tetra-nucleotide microsatellite markers were characterized by 454 XL+ Titanium pyrosequencing. High average levels of allelic diversity and heterozygosity were observed (NA = 16.68; Ne = 9.77; HO = 0.643; HE = 0.858). Significant deviations from HWE, as previously observed in other mollusks, were detected in twelve loci. Linkage disequilibrium was detected among two loci pairs. This set of microsatellite loci will be useful to determine the genetic structure and larval connectivity of P. globosa.
KeywordsTetra-nucleotide microsatellite Panopea Gulf of California Marine connectivity Next generation sequencing
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