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Conservation Genetics Resources

, Volume 6, Issue 3, pp 653–655 | Cite as

Development and characterization of microsatellite loci for the primrose Primula vulgaris and successful cross-amplification in the congeneric P. elatior and P. veris

  • Miyuki M. SeinoEmail author
  • Clara de Vega
  • Pilar Bazaga
  • Hans Jacquemyn
  • Carlos M. Herrera
Microsatellite Letters

Abstract

The perennial herb Primula vulgaris (Primulaceae) faces severe fragmentation of its natural forest habitat in the northern border of its distribution due to intensive agricultural practices and increased urbanisation. In Belgium and northern France, P. vulgaris populations show dramatic declines that could lead to local extinctions. Here we describe 12 novel microsatellite loci obtained by 454-pyrosequencing of a microsatellite-enriched library. Number of alleles per polymorphic locus varied between 2 and 6, and observed heterozygosity ranged from 0.16 to 0.73. Furthermore, sets of 8 loci successfully cross-amplified in the congeneric P. elatior and P. veris. These markers will be of great value for genetic diversity and conservation studies.

Keywords

454-pyrosequencing Fragmentation Microsatellites Primula elatior Primula veris Primula vulgaris 

Notes

Acknowledgments

This work was funded by the Spanish Ministerio de Economía y Competitividad through Grants CGL2010-15964 to CMH, FPI predoctoral Grants BES-2011-046472 and EEBB-I-13-06242 to MMS, and through the Severo Ochoa Programme for Centers of Excellence (SEV-2012-0262) to CdV.

References

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Miyuki M. Seino
    • 1
    Email author
  • Clara de Vega
    • 1
  • Pilar Bazaga
    • 1
  • Hans Jacquemyn
    • 2
  • Carlos M. Herrera
    • 1
  1. 1.Estación Biológica de DoñanaCSICSevillaSpain
  2. 2.Division of Plant Ecology and Systematics, Biology DepartmentKU LeuvenHeverleeBelgium

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