Development and characterization of microsatellite loci for the primrose Primula vulgaris and successful cross-amplification in the congeneric P. elatior and P. veris
The perennial herb Primula vulgaris (Primulaceae) faces severe fragmentation of its natural forest habitat in the northern border of its distribution due to intensive agricultural practices and increased urbanisation. In Belgium and northern France, P. vulgaris populations show dramatic declines that could lead to local extinctions. Here we describe 12 novel microsatellite loci obtained by 454-pyrosequencing of a microsatellite-enriched library. Number of alleles per polymorphic locus varied between 2 and 6, and observed heterozygosity ranged from 0.16 to 0.73. Furthermore, sets of 8 loci successfully cross-amplified in the congeneric P. elatior and P. veris. These markers will be of great value for genetic diversity and conservation studies.
Keywords454-pyrosequencing Fragmentation Microsatellites Primula elatior Primula veris Primula vulgaris
This work was funded by the Spanish Ministerio de Economía y Competitividad through Grants CGL2010-15964 to CMH, FPI predoctoral Grants BES-2011-046472 and EEBB-I-13-06242 to MMS, and through the Severo Ochoa Programme for Centers of Excellence (SEV-2012-0262) to CdV.
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