Abstract
Eight polymorphic microsatellite loci are described for the endangered Amur ratsnake, Elaphe schrenckii (Colubridae). Loci were screened in 31 unrelated individuals of E. schrenckii collected from Liaoning Province, China. These markers revealed a high degree of genetic diversity (6–20 alleles per locus) and heterozygosity (H O ranged from 0.296 to 1.000, and H E ranged from 0.469 to 0.850). No significant linkage disequilibrium between pairs of loci was found, except one of them significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These loci are the first time to be characterized and will be useful for population genetic structure investigation, conservation management and paternity identification study for E. schrenckii.
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Acknowledgments
The work was carried out in compliance with the current laws of China, and was supported by grants from Natural Science Foundation of China (31272294 and 31200283), the Priority Academic Program Development of Jiangsu Higher Education Institutions (CXLX11_0885), the Jiangsu Provincial Foundation of Natural Science (BK2012849) and the NSFC for Talents Training in Basic Science (J1103507). The authors would like to thank Yan-qing Wu for his help during the research.
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Xu, Xy., Fu, Tl., Qu, YF. et al. Isolation and characterization of microsatellite loci in the endangered Amur ratsnake Elaphe schrenckii (Reptilia; Squamata; Colubridae) from China. Conservation Genet Resour 6, 645–646 (2014). https://doi.org/10.1007/s12686-014-0166-z
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DOI: https://doi.org/10.1007/s12686-014-0166-z