Skip to main content
SpringerLink
Log in

Isolation and characterization of twenty-eight polymorphic microsatellite markers in Odontobutis potamophila and cross-amplification in other Gobioidei

  • Microsatellite Letters
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Odontobutis potamophila is widely distributed in river systems of southeast China. Twenty-eight microsatellite loci were isolated and characterized for this species. Number of alleles ranged from 2 to 19 per locus, and the observed and expected heterozygosity were 0.161–0.967 and 0.207–0.928, respectively. No evidence of linkage disequilibrium was detected. The significant departures from Hardy–Weinberg expectations at three loci suggested the presence of null alleles. These microsatellite loci will be useful for studying population genetic variation. Cross-amplification of these microsatellite loci indicated that many are polymorphic in four closely related species of Gobioidei.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Excoffier L, Lischer H (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567

    Article  PubMed  Google Scholar 

  • Huckstorf V (2012) Odontobutis potamophilus. In: IUCN 2012. IUCN red list of threatened species

  • Lalitha S (2000) Primer Premier 5. Biotechnol Softw Internet Rep 6:270–272

    Article  Google Scholar 

  • Oosterhout CV, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

  • Sun XW, Lu CY, Liang LQ (2005) Isolation of micro-satellite with enrichment by magnetic beads grass carp, Ctenopharyngodon idella. J Fish China 29:482–486

    CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the National Spark Program Project (2013GA690166), Jiangsu Province Science and Technology Support Program (BE2013441), Science and Technology Achievement Transformation Fund Project of Nanjing Normal University (2013-02), and the Project Foundation of the Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Author information

Authors and Affiliations

  1. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China

    Fei Zhu, Shaowu Yin, Lijuan Zhang, Xinyuan Hou & Yali Hu

  2. College of Ocean, Hainan University, Haikou, 570228, China

    Fei Zhu & Jian Luo

  3. Co-Innovation Center for Marine Bio-Industry Technology, Lianyungang, 222005, China

    Fei Zhu, Shaowu Yin, Lijuan Zhang, Xinyuan Hou & Yali Hu

Authors
  1. Fei Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jian Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shaowu Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lijuan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xinyuan Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yali Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shaowu Yin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhu, F., Luo, J., Yin, S. et al. Isolation and characterization of twenty-eight polymorphic microsatellite markers in Odontobutis potamophila and cross-amplification in other Gobioidei. Conservation Genet Resour 6, 601–604 (2014). https://doi.org/10.1007/s12686-014-0150-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-014-0150-7

Keywords

Search

Navigation