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Conservation Genetics Resources

, Volume 6, Issue 3, pp 585–587 | Cite as

Isolation via 454 sequencing, and characterisation of microsatellite markers for the Pilbara endemic Acacia atkinsiana (Fabaceae)

  • Esther Levy
  • Margaret Byrne
  • David J. Coates
  • Stephen J. van Leeuwen
  • Shelley McArthur
  • Bronwyn Macdonald
  • Michael G. Gardner
Microsatellite Letters

Abstract

We isolated 11 new polymorphic microsatellite markers from Acacia atkinsiana, a plant species endemic to the Pilbara region in Western Australia. Next generation (454) sequencing was used to identify 28 microsatellite markers that were trialled in individuals across the species range. Characterisation of 11 of these loci in 24 individuals from a single population yielded between two and nine alleles per locus, and observed heterozygosity ranged from 0.042 to 0.958. Four loci that amplified in A. atkinsiana were also successfully amplified and polymorphic in Acacia ancistrocarpa. These loci will be valuable for assessing population genetic structure in A. atkinsiana and identifying seed sources for rehabilitation of disturbed sites.

Keywords

Acacia atkinsiana Microsatellites 454 GS-FLX Shotgun sequencing Revegetation 

Notes

Acknowledgments

We acknowledge the assistance and support of Alison Fitch from Flinders University. This project was funded by Rio Tinto.

References

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Copyright information

© © Crown Copyright as represented by the Government of Western Australia, Department of Parks and Wildlife  2014

Authors and Affiliations

  • Esther Levy
    • 1
  • Margaret Byrne
    • 1
  • David J. Coates
    • 1
  • Stephen J. van Leeuwen
    • 1
  • Shelley McArthur
    • 1
  • Bronwyn Macdonald
    • 1
  • Michael G. Gardner
    • 2
    • 3
    • 4
  1. 1.Science Division, Department of Parks and WildlifeBentley Delivery CentreBentleyAustralia
  2. 2.School of Biological SciencesFlinders UniversityAdelaideAustralia
  3. 3.Australian Centre for Evolutionary Biology and BiodiversityUniversity of AdelaideAdelaideAustralia
  4. 4.Evolutionary Biology UnitSouth Australian MuseumAdelaideAustralia

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