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Characterization and cross-species amplification of 11 polymorphic microsatellite loci in Liobagrus marginatoides

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Abstract

Liobagrus marginatoides is endemic to the upper Yangtze River basin in China. By using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol, we identified eleven microsatellite loci in a wild population. The number of alleles per locus ranged from 4 to 10. The observed and expected heterozygosity ranged from 0.3258 to 1.000 and from 0.6506 to 0.8712, respectively. Cross-amplification was tested in related species L. nigricauda, L. marginalus and L. kingi.

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Acknowledgments

This study was supported by the project of China Three Gorges Corporation (0799527).

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Authors and Affiliations

  1. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, 1st Wudayuan Road, East Lake Hi-Tech Development Zone, Wuhan, 430223, Hubei, People’s Republic of China

    Xiang-Yang Jia, Deng-Qiang Wang, Hui-Wu Tian & Da-Qing Chen

  2. College of Life Sciences, Chongqing Normal University, Chongqing, 401331, People’s Republic of China

    Xiang-Yang Jia & Ying-Wen Li

  3. Xianning No. 1 People’s Hospital, Xianning, 437100, People’s Republic of China

    Xiang-Yang Jia & Bin Tu

Authors
  1. Xiang-Yang Jia
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  2. Ying-Wen Li
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  3. Deng-Qiang Wang
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  4. Hui-Wu Tian
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  5. Bin Tu
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  6. Da-Qing Chen
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Correspondence to Da-Qing Chen.

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Jia, XY., Li, YW., Wang, DQ. et al. Characterization and cross-species amplification of 11 polymorphic microsatellite loci in Liobagrus marginatoides . Conservation Genet Resour 5, 1087–1089 (2013). https://doi.org/10.1007/s12686-013-9992-7

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  • DOI: https://doi.org/10.1007/s12686-013-9992-7

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