Conservation Genetics Resources

, Volume 5, Issue 4, pp 1157–1158 | Cite as

Isolation and characterization of 20 microsatellite loci for the saltmarsh plant Triglochin maritima L.

  • Romuald Rouger
  • Alistair S. JumpEmail author
Microsatellite Letters


Twenty microsatellite markers were developed for the polyploid plant Triglochin maritima L., an important component of declining saltmarsh ecosystems that are now subject to much restoration effort. All loci were polymorphic when tested across 24 individuals from three populations. The average number of alleles per population was 6, ranging from 2 to 12. Private alleles were identified in each population, demonstrating the utility of these markers for the investigation of the population genetic structure and diversity of this species.


454 Sequencing Polyploid Saltmarsh Restoration Halophyte 



This work was supported by the Esmée Fairbairn foundation, the University of Stirling and the Royal Society for the Protection of Birds. We thank Mario Vallejo-Marin and Olivier Lepais for technical advice.

Supplementary material

12686_2013_9986_MOESM1_ESM.pdf (298 kb)
Supplementary material 1 (PDF 299 kb)


  1. Davy AJ, Bishop GF (1991) Triglochin maritima L. J Ecol 79:531–555CrossRefGoogle Scholar
  2. Lambracht E, Westberg E, Kadereit JW (2007) Phylogeographic evidence for the postglacial colonization of the North and Baltic Sea coasts from inland glacial refugia by Triglochin maritima L. Flora 202:79–88CrossRefGoogle Scholar
  3. Lepais O, Bacles CFE (2011) Comparison of random and SSR-enriched shotgun pyrosequencing for microsatellite discovery and single multiplex PCR optimization in Acacia harpophylla F. Muell. Ex Benth. Mol Ecol Resour 11:711–724PubMedCrossRefGoogle Scholar
  4. Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin JF (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26:403–404PubMedCrossRefGoogle Scholar
  5. Schuelke M (2000) An economic method for the fluorescent labelling of PCR fragments. Nat Biotechnol 18:233–234PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Biological and Environmental Sciences, School of Natural SciencesUniversity of StirlingStirlingUK

Personalised recommendations