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Isolation and characterisation of 14 microsatellite loci from a short-range endemic, Western Australian tree, Banksia arborea (C.A. Gardner)

  • Microsatellite Letters
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Abstract

Fourteen nuclear microsatellite markers were developed for the short-range endemic tree, Banksia arborea to examine patterns of genetic diversity and structure across the species’ restricted distribution. One hexanucleotide, three trinucleotide and 10 dinucleotide repeat loci were developed and tested on 24 individuals from one population. The number of alleles per locus ranged from 2 to 11. Observed and expected heterozygosity ranged from 0 to 0.833 and 0.044 to 0.823 respectively. All loci showed independent inheritance and null alleles are suspected to occur in four loci.

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Acknowledgments

These microsatellite markers were produced using funding from the Holsworth Wildlife Research Endowment. We thank S. McArthur, B. Macdonald and K. Ottewell for advice regarding DNA extraction and amplification, and E. Ager for collection of samples.

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Authors and Affiliations

  1. School of Animal Biology, The University of Western Australia, M092, 35 Stirling Hwy, Crawley, WA, 6009, Australia

    Heidi Nistelberger & J. Dale Roberts

  2. Science Division, Department of Environment and Conservation, Bentley Delivery Centre, Locked Bag 104, Bentley, WA, 6983, Australia

    David Coates & Margaret Byrne

Authors
  1. Heidi Nistelberger
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  2. J. Dale Roberts
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  3. David Coates
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  4. Margaret Byrne
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Correspondence to Heidi Nistelberger.

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Nistelberger, H., Dale Roberts, J., Coates, D. et al. Isolation and characterisation of 14 microsatellite loci from a short-range endemic, Western Australian tree, Banksia arborea (C.A. Gardner). Conservation Genet Resour 5, 1143–1145 (2013). https://doi.org/10.1007/s12686-013-9983-8

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  • DOI: https://doi.org/10.1007/s12686-013-9983-8

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