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Development and characterisation of ten polymorphic microsatellite markers for the pine-tree lappet moth Dendrolimus pini (Lepidoptera: Lasiocampidae)

Abstract

A total of ten novel microsatellite markers developed for the pine-tree lappet moth (Dendrolimus pini) are presented. The loci produce clear polymorphic banding patterns, exhibiting between 6 and 18 alleles, with observed heterozygosity values ranging from 0.6190 to 0.9524. These markers will be used to study the genetic diversity and population structure of a recently identified D. pini breeding population in the UK. The microsatellites were also tested in Dendrolimus superans and Dendrolimus sibiricus and produced clear banding patterns within the expected product size range and may therefore have utility in these species.

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References

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Acknowledgments

The authors would like to thank the Sachsen-Anhalt forest service, Germany, for providing the caterpillar samples.

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Correspondence to Stuart A’Hara.

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A’Hara, S., Cottrell, J. Development and characterisation of ten polymorphic microsatellite markers for the pine-tree lappet moth Dendrolimus pini (Lepidoptera: Lasiocampidae). Conservation Genet Resour 5, 1135–1137 (2013). https://doi.org/10.1007/s12686-013-9977-6

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Keywords

  • Dendrolimus pini
  • Moth
  • Lepidoptera
  • Microsatellite marker
  • Lasiocampidae
  • Population genetics