Abstract
The Asian Stubtail Urosphena squameiceps winters in Southeast Asia which is facing a serious threat from deforestation and the species is categorized as a threatened species at some of the breeding populations. Monitoring population dynamics of the species in the field is difficult because they are secretive and prefer dense undergrowth. Therefore, developing genetic markers from the species is useful for detecting appropriate conservational management units and to understanding their unique breeding system. We isolated and characterized 13 microsatellite loci of the Asian Stubtail by using a next-generation sequencer. The number of alleles and the expected heterozygosity at each locus were 2–26 (mean 10.31) and 0.14–0.92 (mean 0.64), respectively. The combined first-parent non-exclusion probability was <0.005.
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Acknowledgments
We thank N. Kawaji for fieldwork assistance and C. Adenyo for experimental assistance and C. Barnett for English correction. The Roche GS Junior Sequencer was operated by the Cooperation Research Program of the Wildlife Research Center, Kyoto University. This study was supported by a Grant-in-Aid for JSPS Fellows.
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Kamioki, M., Ando, H., Isagi, Y. et al. Development of microsatellite markers for the Asian Stubtail Urosphena squameiceps by using next-generation sequencing technology. Conservation Genet Resour 5, 1027–1029 (2013). https://doi.org/10.1007/s12686-013-9960-2
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DOI: https://doi.org/10.1007/s12686-013-9960-2