Abstract
The venus clam Cyclina sinensis is one of the most commercially important species in marine clam aquaculture on the coast of China. In this study, 11 novel polymorphic microsatellite loci were isolated and characterized from a wild stock of the venus clam found on the Dalian coast of China. The number of alleles varied between three and six, and the observed and expected heterozygosity at the population level ranged from 0.8276 to 1.0000–0.6672 to 0.7876, respectively. No single locus deviated significantly from Hardy–Weinberg equilibrium (P > 0.01).These microsatellite markers will be useful in studies of parentage, population genetics and genome mapping in this species.
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Acknowledgments
This study was supported in part by grants from the Science and Technology Development Project of Lianyungang City (Grant No. CN0906), Jiangsu Key Laboratory of Marine Biotechnology Project (Grant No. 2007HS004), the Central Finance to support the Development of Local Universities Discipline of Applied Marine Biology for Jiangsu Province (Grant No. CXTD26) and the Project of Key Laboratory of Freshwater Fisheries Genetic Resources and Utilization of the Agriculture Ministry of China (Grant No. KFT2012-1).
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Dong, Z., Li, X., You, X. et al. Eleven novel polymorphic microsatellite markers for genetic analysis of the venus clam Cyclina sinensis Gmelin, 1791. Conservation Genet Resour 5, 1019–1021 (2013). https://doi.org/10.1007/s12686-013-9958-9
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DOI: https://doi.org/10.1007/s12686-013-9958-9