Abstract
We isolated 315 sequences from a Phelsuma guimbeaui microsatellite-enriched genomic library. Primer sets were designed for 44 loci and used to genotype 29 unrelated individuals belonging to a population in the west of Mauritius. All the loci were polymorphic and the number of alleles ranged from 6 to 34. Mean observed and expected heterozygosity varied from 0.29 to 1.00 and 0.48 to 0.97, respectively. Thirteen loci displayed evidence of deviation from Hardy–Weinberg equilibrium, of which 11 also had an estimated null allele frequency ≥10 %. The microsatellite loci will be used to evaluate the population structure and genetic diversity of P. guimbeaui in Mauritius.
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Acknowledgments
This study was funded by the UK Natural Environment Research Council (NERC), University of Bristol, a Rufford Small Grant, Interessengruppe Phelsuma and private sponsors. Mannickchand Puttoo, Vishnuduth Bachraz and Kevin Ruhomaun from the National Parks and Conservation Service (Mauritius) gave constant support during the course of the project. The Mauritian Wildlife Foundation also supplied logistic support and facilities. The laboratory work was conducted at the NERC Biomolecular Analysis Facility at the University of Sheffield. We thank Terry Burke, Clemens Küpper, Cheryl Mills, Ralph Budzinski and Caitriona McInerney for helpful discussions.
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Buckland, S., Horsburgh, G.J., Dawson, D.A. et al. Isolation and characterisation of Mauritius lowland day gecko Phelsuma guimbeaui microsatellite loci. Conservation Genet Resour 5, 1013–1018 (2013). https://doi.org/10.1007/s12686-013-9957-x
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DOI: https://doi.org/10.1007/s12686-013-9957-x