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Characterization of 9 polymorphic microsatellite loci in Lamellibrachia sp. 2, a tubeworm found at deep-sea hydrothermal vents and cold seeps


Lamellibrachia sp. 2 is a deep-sea vestimentiferan tubeworm found at hydrothermal vents on the Mid-Cayman Spreading Center and at hydrocarbon seeps in the Gulf of Mexico and off the coast of Barbados. Nine selectively neutral and unlinked polymorphic microsatellite loci were developed for this species. Eight of these loci conformed to Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.14 to 0.92. Microsatellites developed for Lamellibrachia sp. 2 are being deployed to study connectivity and gene flow among populations of this species.

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  • Balloux F, Lugon-Moulin N (2002) The estimation of population differentiation with microsatellite markers. Mol Ecol 11(2):155–165. doi:10.1046/j.0962-1083.2001.01436.x

    PubMed  Article  Google Scholar 

  • Doyle JJ, Dickson E (1987) Preservation of plant samples for DNA restriction endonuclease analysis. Taxon 36:715–722

    Article  Google Scholar 

  • Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94

    PubMed  Article  CAS  Google Scholar 

  • Foll M, Gaggliotti OE (2008) A genome scan method to identify selected loci appropriate for both dominant and codominant markers: a Bayesian perspective. Genetics 180:977–993

    PubMed  Article  Google Scholar 

  • Jacobson A, Plouviez S, Thaler AD, Van Dover CL (2013) Characterization of 13 polymorphic microsatellite loci in Rimicaris hybisae, a shrimp from deep-sea hydrothermal vents. Conservation Genet Resour. 5(2):449–451. doi:10.1007/s12686-012-9825-0

  • Kalinowski ST (2006) HW-QuickCheck: an easy-to-use computer program for checking genotypes for agreement with Hardy-Weinberg expectations. Mol Ecol Notes 6:974–979

    Article  Google Scholar 

  • Miglietta MP, Hourdez S, Cowart DA, Schaeffer SW, Fisher C (2010) Species boundaries of Gulf of Mexico vestimentiferans (Polychaeta, Siboglinidae) inferred from mitochondrial genes. Deep-Sea Res II 57:1916–1925

    Article  Google Scholar 

  • Plouviez S, Shank TM, Faure B, Daguin-Thiébaut C, Viard F, Lallier FH, Jollivet D (2009) Comparative phylogeography among hydrothermal vent species along the East Pacific Rise reveals vicariant processes and population expansion in the South. Mol Ecol. doi:10.1111/j.1365-294X.2009.04325.x

    PubMed  Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43(1):223–225

    Article  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa

    Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234

    PubMed  Article  CAS  Google Scholar 

  • Southward EC, Anderson AC, Hourdez S (2011) Lamellibrachia anaximandri n. sp., a new vestimentiferan tubeworm (Annelida) from the Mediterranean, with notes on frenulate tubeworms from the same habitat. Zoosystema 33(3):245–279

    Article  Google Scholar 

  • Van Dover CL, German CR, Speer KG, Parson LM, Vrijenhoek RC (2002) Evolution and biogeography of deep-sea vent and seep invertebrates. Science 295(5558):1253–1257. doi:10.1126/science.1067361

    PubMed  Article  Google Scholar 

  • van Oosterhout C, Hutchinson W, Willds D, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

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This research was supported by NSF award OCE-1031050 and NASA ASTEP subcontract through WHOI (NNX09AB75G) to CLVD. We are thankful to the Chief Scientist, C. German, the crew of the R/V Atlantis (AT18-16) and the crew of the ROV Jason. We also acknowledge Mengyou Wu for help with DNA extractions. SP, ADT, CLVD conceived the study; SP, CLVD collected samples, SP and AJ undertook molecular benchwork; AJ, SP, ADT analyzed the data; AJ, SP, ADT, and CLVD drafted the manuscript.

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Correspondence to Alixandra Jacobson.

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Jacobson, A., Plouviez, S., Thaler, A.D. et al. Characterization of 9 polymorphic microsatellite loci in Lamellibrachia sp. 2, a tubeworm found at deep-sea hydrothermal vents and cold seeps. Conservation Genet Resour 5, 1005–1007 (2013).

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  • Mid-Cayman Spreading Center
  • Von Damm vent field
  • Barbados seeps
  • Gulf of Mexico seeps
  • Vestimentifera
  • Gene flow
  • Connectivity