Development and characterization of 22 microsatellite loci for the ringed salamander (Ambystoma annulatum) using paired-end Illumina shotgun sequencing
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We isolated and screened 150 microsatellite loci from the ringed salamander, Ambystoma annulatum, an ambystomatid salamander endemic to the Interior Highlands. Twenty-two polymorphic loci were identified and pooled into two multiplex reactions. These loci will be valuable tools for assessing population genetic structure and connectivity across the landscape, and informing management of this species.
KeywordsAmbystoma Population genetics Landscape genetics Multiplex PCR PAL_Finder
We thank S. Lance (University of Georgia) for Illumina sequencing and processing of reads. This research was funded by the DoD Strategic Environmental Research and Development Program (RC2155).
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