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Conservation Genetics Resources

, Volume 5, Issue 4, pp 993–995 | Cite as

Development and characterization of 22 microsatellite loci for the ringed salamander (Ambystoma annulatum) using paired-end Illumina shotgun sequencing

  • William E. PetermanEmail author
  • Luke R. Pauley
  • Emily R. Brocato
  • Elsa C. Stuart
  • Raymond D. Semlitsch
  • Lori S. Eggert
Technical Note

Abstract

We isolated and screened 150 microsatellite loci from the ringed salamander, Ambystoma annulatum, an ambystomatid salamander endemic to the Interior Highlands. Twenty-two polymorphic loci were identified and pooled into two multiplex reactions. These loci will be valuable tools for assessing population genetic structure and connectivity across the landscape, and informing management of this species.

Keywords

Ambystoma Population genetics Landscape genetics Multiplex PCR PAL_Finder 

Notes

Acknowledgments

We thank S. Lance (University of Georgia) for Illumina sequencing and processing of reads. This research was funded by the DoD Strategic Environmental Research and Development Program (RC2155).

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • William E. Peterman
    • 1
    Email author
  • Luke R. Pauley
    • 1
  • Emily R. Brocato
    • 1
  • Elsa C. Stuart
    • 1
  • Raymond D. Semlitsch
    • 1
  • Lori S. Eggert
    • 1
  1. 1.Division of Biological SciencesUniversity of Missouri-ColumbiaColumbiaUSA

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