Development and characterization of 18 microsatellite loci for the spotted salamander (Ambystoma maculatum) using paired-end Illumina shotgun sequencing
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We isolated and screened 68 microsatellite loci from the spotted salamander, Ambystoma maculatum, in Missouri, USA. Eighteen polymorphic loci were identified and pooled into two multiplex reactions. These loci are a needed addition to the known loci for A. maculatum, which were developed in a different geographic region and are unsuitable for population or landscape genetic analyses in Missouri. To effectively protect and manage this species, regionally-relevant studies of population genetic structure and connectivity are necessary.
KeywordsAmbystoma Population genetics Landscape genetics Multiplex PCR PAL_Finder
We thank S. Lance (University of Georgia) for Illumina sequencing and processing of reads. This research was funded by the DoD Strategic Environmental Research and Development Program (RC2155).
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