Abstract
We isolated and characterized the first polymorphic microsatellite for the Brazilian and Hooded slipper lobsters species (Scyllarides brasiliensis and S. deceptor). Thirteen polymorphic loci (2–31 alleles/locus, Ho = 0.056–0.975, He = 0.155–0.958), were characterized in S. brasiliensis (N = 40). Twelve polymorphic loci (3–22 alleles/locus, Ho = 0.333–0.900, He = 0.337–0.940), were characterized in S. deceptor (N = 30) from different localities on the Brazilian coast. These loci were also tested in four scyllarid lobsters, Scyllarides aequinoctialis, S. delfosi, Scyllarus depressus and Parribacus antarcticus.
References
Bloor PA, Barker FS, Watts PC, Noyes HA, Kemp SJ (2001) Microsatellite libraries by enrichment. http://www.genomics.liv.ac.uk/animal/MICROSAT.PDF. Accessed 31 March 2013
de Arruda MP, Gonçalves EC, Schneider MP, da Silva AL, Morielle-Versute E (2010) An alternative genotyping method using dye-labeled universal primer to reduce unspecific amplifications. Mol Biol Rep 37:2031–2036
Duarte LFA, Severino-Rodrigues E, Gasalla MA (2010) Slipper lobster (Crustacea, Decapoda, Scyllaridae) fisheries off the southeastern coast of Brazil: I. Exploitation patterns between 23°00′ and 29°65′ S. Fish Res 102:141–151
Groeneveld JC, Goñi R, Latrouite D (2006) Palinurus species. In: Phillips BF (ed) Lobsters: biology, management, aquaculture and fisheries. Blackwell Publishing Ltd, Oxford, pp 385–411
Lavalli KL, Spanier E (2007) Introduction to the biology and fisheries of slipper lobsters. In: Lavalli KL, Spanier E (eds) The biology and fisheries of slipper lobster. CRC Press, Florida, pp 3–24
Martins WS, Lucas DCS, Neves KFS, Bertioli DJ (2009) WebSat—a web software for microsatellite marker development. Bioinformation 3:282–283
Miller SA, Dykes DD, Polesky HF (1988) A simple salting procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16:215
Phillips BF, Melville-Smith R (2006) Panulirus species. In: Phillips BF (ed) Lobsters: biology, management, aquaculture and fisheries. Blackwell Publishing Ltd, Oxford, pp 359–384
Raymond M, Rousset F (1995) GENEPOP version 1.2: population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Rice WR (1989) Analyzing tables of statistical test. Evolution 43:223–225
Salin F (2010) Autobin v0.9. http://www4.bordeaux-aquitaine.inra.fr/biogeco/Ressources/Logiciels/Autobin. Accessed 31 March 2013
Santos MCF, Freitas AETS (2002) Estudo sobre a lagosta sapata Scyllarides brasiliensis Rathbun, 1906 (Crustacea: Decapoda: Scyllaridae) no litoral dos estados de Pernambuco e Alagoas—Brasil. Bol Técn Cient CEPENE 10:123–143
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234
Spanier E, Lavalli KL (2007) Slipper lobster fisheries—present status and future perspectives. In: Lavalli KL, Spanier E (eds) The biology and fisheries of the slipper lobster. CRC Press, Florida, pp 377–391
van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Acknowledgments
We thank N. P. Cavaleiro and A. V. Vasconcellos for assistance during microsatellite development. Financial support was provided by Brazilian Ministries for Fisheries (MPA) and Science (MCTI), CNPq, CAPES, and FAPERJ.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rodríguez-Rey, G.T., Cunha, H.A., Lazoski, C. et al. Polymorphic microsatellite loci from Brazilian and Hooded slipper lobsters (Scyllarides brasiliensis and S. deceptor), and cross-amplification in other scyllarids. Conservation Genet Resour 5, 985–988 (2013). https://doi.org/10.1007/s12686-013-9949-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-013-9949-x