Abstract
We describe the isolation of 12 tetranucleotide microsatellite loci from the African Yellow White-eye (Zosterops senegalensis) using an enrichment protocol. All loci were variable with the number of alleles ranging from 2 to 13, and observed heterozygosity ranging from 0.333 to 1.000. All 12 loci were also screened on a panel of three White-eye taxa and were observed to be polymorphic. Given that the Orange River (Z. pallidus) and Cape White-eyes (Z. virens) differ by 3.65 % in mitochondrial DNA, we expect the isolated loci to be broadly applicable to studies attempting to resolve the many taxonomic difficulties that have arisen given the rapid and recent evolutionary radiation represented by members of the Zosteropidae, particularly among the many species which are of conservation concern given their restricted geographic distributions.
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Acknowledgments
Microsatellite enrichment was carried out in the Pritzker Laboratory for Molecular Systematics and Evolution operated with support from the Pritzker Foundation. We thank the southern African provincial authorities for granting sampling permits. Fieldwork was supported in part by grants from NSF (DEB-1120356, DEB-1119931) to RCKB and GV, respectively. This is publication number 1456 of the Biodiversity Research and Teaching Collections at Texas A&M.
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Oatley, G., Feldheim, K.A., Voelker, G. et al. Development of twelve tetranucleotide microsatellite loci for White-eyes (Zosterops spp.) for use in phylogeographic and hybridization studies. Conservation Genet Resour 5, 977–979 (2013). https://doi.org/10.1007/s12686-013-9947-z
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DOI: https://doi.org/10.1007/s12686-013-9947-z