Abstract
Thirty-six primer pairs were designed for the detection of rare aquatic species from environmental DNA samples, targeting species-specific binding sequences within the ‘barcoding’ segment of the mitochondrial cytochrome oxidase I gene. Cross-species amplification tests confirmed the specificity of 18 of 26 tested primer pairs. These primers will be a useful tool for targeted detection of endangered and invasive aquatic species of concern to the Laurentian Great Lakes and help to inform management responses.
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Acknowledgments
Ontario Ministry of Natural Resources (OMNR) and the Canada-Ontario Agreement for Great Lakes Ecosystem (COA) provided funding. Samples provided by Kelly Bowen, Ron Dermott, Michael Fox, Bill Glass, Tim Johnson, Joseph Love, Andrew Mahon, John Odenkirk, OMNR Biodiversity Section and Lake Ontario Management Unit, Kirby Punt, Scott Reid, and the Royal Ontario Museum.
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Bronnenhuber, J.E., Wilson, C.C. Combining species-specific COI primers with environmental DNA analysis for targeted detection of rare freshwater species. Conservation Genet Resour 5, 971–975 (2013). https://doi.org/10.1007/s12686-013-9946-0
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DOI: https://doi.org/10.1007/s12686-013-9946-0