Abstract
Eight polymorphic microsatellites in the gray triggerfish, Balistes capriscus, are presented. For 24 genotyped individuals, the number of observed alleles per locus ranged from 10 to 21 (mean = 15), and observed heterozygosity varied between 0.708 and 1.000. Genotypic frequencies did not deviate significantly from Hardy–Weinberg equilibrium proportions, and there was no evidence of null alleles or linkage disequilibrium among loci. These microsatellite loci are additional markers for fisheries managers to utilize in population-level B. capriscus assessments.
References
Antoni L, Saillant EA (2012) Development and characterization of microsatellite markers in the gray triggerfish (Balistes capriscus). Conserv Genet Resour 4:629–631
Antoni L, Emerick N, Saillant E (2011) Genetic variation of gray triggerfish in US waters of the Gulf of Mexico and Western Atlantic Ocean as inferred from mitochondrial DNA sequences. N Am J Fish Manage 31:714–721
Chapuis MP, Estoup A (2007) Microsatellite null alleles and estimation of population differentiation. Mol Ecol Evol 24:621–631
Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Res 10:564–567
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222
Harper D, McClellan D (1997) A review of the biology and fishery for gray triggerfish, Balistes capriscus, in the Gulf of Mexico. National Marine Fisheri9es Service, Southeast Fisheries Science Center, Miami Laboratory Contribution MIA-96/97-52, Miami, Florida
Litt M, Hauge X, Sharma V (1993) Shadow bands seen when typing polymorphic dinucleotide repeats—some causes and cures. Biotechniques 15:280–284
Rosen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa
Sazonov YG, Galaktionova AI (1987) Some data on the morphometrics of the gray triggerfish, Balistes carolinensis, of the central-eastern Atlantic. J Ichthyol 27:173–176
Sorenson L, McDowell JR, Graves JE (2011) Isolation and characterization of microsatellite markers for blue marlin, Makaira nigricans. Conserv Genet Resour 3:721–723
Valle M, Legault CM, Ortiz M (2001) A stock assessment for gray triggerfish, Balistes capriscus, in the Gulf of Mexico. Sustainable fisheries division contribution SFD-00/01-124, NOAA Fisheries, Department of Commerce, Miami, Florida
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Acknowledgments
I would like to acknowledge Jan R. McDowell (VIMS), John E. Graves (VIMS), Brian Bowen (UH), Jessica Lynch Alfaro (UCLA), and Michael E. Alfaro (UCLA) for lab support. Many thanks to Bill Bogue and Danny Davis for collecting tissues. This research was supported by a University of California, Los Angeles, Ecology and Evolutionary Biology research award.
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Sorenson, L. Isolation and characterization of novel microsatellite markers in the gray triggerfish, Balistes capriscus . Conservation Genet Resour 5, 967–969 (2013). https://doi.org/10.1007/s12686-013-9945-1
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DOI: https://doi.org/10.1007/s12686-013-9945-1