Abstract
Understanding patterns of contemporary population genetic structure and diversity is critically important for cold-adapted Southern Ocean benthic species along the Scotia Arc to distinguish between natural and anthropogenically-mediated range expansions and detect population declines due to the rapid climate change. We report the isolation and characterization of 10 polymorphic microsatellite loci for the Antarctic endemic giant isopod Glyptonotus antarcticus from the Eastern Scotia Arc (n = 30). The number of alleles per locus ranged from 3 to 11 with no evidence of significant linkage disequilibrium between loci. These markers will prove useful in studying the fine-scale processes, which have shaped the spatial distribution of variation, and hypothesis-testing in the Scotia Arc region of Antarctica.
This is a preview of subscription content, log in via an institution to check access.
References
Aronson RB, Thatje S, McClintock JB, Hughes KA (2011) Anthropogenic impacts on marine ecosystems in Antarctica. Ann N Y Acad Sci 1223:82–107
Beerli P, Palczewski M (2010) Unified framework to evaluate panmixia and migration direction among multiple sampling locations. Genetics 185:313–326
Held C, Leese F (2007) The utility of fast evolving molecular markers for studying speciation in the Antarctic benthos. Polar Biol 30:513–521
Kraemer L, Beszteri B, Gäbler-Schwarz S, Held C, Leese F, Mayer C, Pöhlmann K, Frickenhaus S (2009) STAMP: extensions to the STADEN sequence analysis package for high throughput interactive microsatellite marker design. BMC Bioinformatics 10:41
Meredith MP, King JC (2005) Rapid climate change in the ocean west of the Antarctic Peninsula during the second half of the 20th century. Geophys Res Lett 32:L19604
Nolte AW, Stemshorn KC, Tautz D (2005) Direct cloning of microsatellite loci from Cottus gobio through a simplified enrichment procedure. Mol Ecol Notes 5:628–636
Peakall ROD, Smouse PE (2005) GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295
Peakall R, Smouse P (2012) GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research–an update. Bioinformatics 28:2537–2539
Peck LS, Convey P, Barnes DKA (2007) Environmental constraints on life histories in antarctic ecosystems: tempos, timings and predictability. Biol Rev 81(1):75–109
Rousset F (2008) Genepop’007: a complete re-implementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106
van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Acknowledgments
We thank Andrea Eschbach for technical assistance. This research was funded by the German Research Foundation (DFG) grant HE 3391/5-1. Emma L. Leach was funded was by a RISE scholarship through German Academic Exchange Service (DAAD).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Agrawal, S., Leach, E.L., Leese, F. et al. Isolation and characterization of 10 polymorphic loci for the giant Antarctic isopod, Glyptonotus antarcticus . Conservation Genet Resour 5, 963–965 (2013). https://doi.org/10.1007/s12686-013-9944-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-013-9944-2