Abstract
Thirteen microsatellite DNA markers were developed from a DNA library of Odorrana margaretae. To characterize these loci, one population from O. margaretae (N = 24) and another from O. graminea (N = 42) were genotyped. All loci were variable, with the number of alleles ranging from 1 to 14 within populations. The average observed and expected heterozygosity per locus ranged from 0.00 to 0.83 and from 0.00 to 0.87, respectively. These markers are readily cross-amplified between the two species and are likely amplifiable in other species of the genus Odorrana. Odorous frogs have a very high level of cryptic species diversity, and we expect that these markers will offer useful tools in species delineation as well as for population genetic study in this ecologically important group.
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This project is supported by the National Natural Science Foundation of China (Grant 31172061).
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Gao, H., Qiao, L., Yang, W. et al. Isolation and characterization of 13 microsatellite DNA loci for the odorous frog Odorrana margaretae and O. graminea (Anura: Ranidae). Conservation Genet Resour 5, 935–937 (2013). https://doi.org/10.1007/s12686-013-9936-2
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DOI: https://doi.org/10.1007/s12686-013-9936-2