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Isolation and characterization of 113 polymorphic microsatellite loci for the Tibetan frog (Nanorana parkeri) using next generation sequencing

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Abstract

We developed and characterized 113 polymorphic microsatellites in the Tibetan frog (Nanorana parkeri) using 454 GS-FLX next generation sequencing technology. These loci were tested in 46 individuals from two N. parkeri populations from the Tibetan plateau. The average number of alleles per locus was 8.09 (range = 2–20). The average observed heterozygosities (H O) per locus in the two populations were 0.58 (range = 0.04–1) and 0.29 (range = 0–0.87), respectively. These microsatellites will be useful for population and conservation genetics studies of N. parkeri and other closely related species.

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References

  • Benson G (1999) Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27:573–580

    Article  PubMed  CAS  Google Scholar 

  • Hu S, Fei L, Hu Q, Huang Q, Huang Y, Jiang Y, Tian W, Ye C, Zhao E (1987) Amphibia-reptilia of Xizang. Science Press, Beijing

    Google Scholar 

  • Ma XY, Lu X, Merilä J (2009) Altitudinal decline of body size in a Tibetan frog. J Zool 279:364–371

    Article  Google Scholar 

  • Park SDE (2001) Trypanotolerance in West African cattle and the population genetic effects of selection. [Ph.D. thesis], University of Dublin, Dublin, Ireland

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225

    Article  Google Scholar 

  • Rousset F (2008) Genepop’ 007: a complete re-implementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer3 on the world wide web for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386

  • Sambrook J, Russell DW (2001) Molecular cloning: A laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Zhang LX, Ma XY, Jiang JP, Lu X (2012) Stronger condition dependence in female size explains altitudinal variation in sexual size dimorphism of a Tibetan frog. Biol J Linn Soc 107:558–565

    Article  Google Scholar 

Download references

Acknowledgments

We thank Le Yang from the Tibet Plateau Biology Research Institute for help in sampling. Our research was supported by grants from the Chinese Academy of Sciences (the 100-talent Program of the Chinese Academy of Sciences; M-HL) and the National Natural Science Foundation of China (No. 31272303; QZ).

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Authors and Affiliations

  1. Key Lab of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China

    Cuimin Wang, Xiaoju Hu, Xiangmo Xie, Pengfei Liu, Qiong Zhang & Meng-Hua Li

  2. College of Life Sciences, University of the Chinese Academy of Sciences, Beijing, 100049, China

    Cuimin Wang, Xiaoju Hu & Pengfei Liu

  3. College of Life Science, Yangtze University, Jingzhou, 434025, Hubei, China

    Xiangmo Xie

  4. Ecological Genetics Research Unit, Department of Biosciences, University of Helsinki, P.O. Box 65, Helsinki, Finland

    Juha Merilä

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  1. Cuimin Wang
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  2. Xiaoju Hu
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  3. Xiangmo Xie
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  4. Pengfei Liu
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  5. Juha Merilä
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  6. Qiong Zhang
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  7. Meng-Hua Li
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Corresponding authors

Correspondence to Qiong Zhang or Meng-Hua Li.

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Wang, C., Hu, X., Xie, X. et al. Isolation and characterization of 113 polymorphic microsatellite loci for the Tibetan frog (Nanorana parkeri) using next generation sequencing. Conservation Genet Resour 5, 915–924 (2013). https://doi.org/10.1007/s12686-013-9932-6

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  • DOI: https://doi.org/10.1007/s12686-013-9932-6

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