Abstract
Twelve highly polymorphic microsatellite markers were isolated and characterized for the frog Yerana yei. Loci were isolated from a genomic library from Y. yei enriched for (ATAG)n repetitive elements. We designed primers that reliably amplified twelve polymorphic loci and tested them on 32 individuals from two populations. The number of alleles per locus ranged from 3–20, the observed and expected heterozygosity ranged within 0.500–1.000 and 0.752–0.964 respectively, and the polymorphic information content (PIC) value ranged from 0.683–0.930. These microsatellite markers could be valuable in future research concerning the conservation genetics and molecular ecology of this species.
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Acknowledgments
The work was supported by grants from The Anhui provincial Outstanding Youth Fund, the Foundation for Young and Key Teachers Project (2011–2014), and the Talent Cultivation Project of Anhui University (02203104/04). We thank Xiaoxue Zhu, Xin Huang and Tao Song for their help in sample collection and lab work.
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Ling Ding and Hui Wang equally contributed to this article.
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Ding, L., Wang, H., Pan, T. et al. Isolation and characterization of twelve polymorphic tetranucleotide microsatellite loci from Yerana yei . Conservation Genet Resour 5, 899–901 (2013). https://doi.org/10.1007/s12686-013-9928-2
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DOI: https://doi.org/10.1007/s12686-013-9928-2