Abstract
Eighteen polymorphic microsatellite primers were developed for Schizopygopsis younghusbandi using an enrichment genomic library and evaluated for PCR using the genomic DNA of 29 individuals from the Lhasa River in Tibet. Eighteen of 70 loci amplified reliable bands revealed a useful degree of polymorphism with a typical multi-banded pattern of polyploid species. The total number of bands per locus ranged from 3 to 30. Average A i and H o were 3.563 and 0.916, respectively. The heterozygosity expected varied from 0.595 to 0.946 (RCeS) and from 0.580 to 0.923 (RCdS), respectively. These microsatellite primers would be useful in population genetics studies for this species and related species of genus Schizopygopsis.
This is a preview of subscription content, log in via an institution to check access.
References
Bloor PA, Barker FS, Watts PC, Noyes HA, Kemp SJ (2001) Microsatellite libraries by enrichment. Protocol available at: http://www.genomics.liv.ac.uk/animal/RESEARCH/MICROSAT.PDF
Chen Y, Cao W (2000) Schizothoracinae. Fauna Sinica, Osteichthyes, Cypriniformes Vol 3 (ed. by P. Yue), Science Press, Beijing, pp. 273–390 (in Chinese)
Chen F, Chen Y (2010) Investigation and protection strategies of fishes of Lhasa river. Acta Hydrobiol Sin 34:278–285 (in Chinese)
Chen F, Chen Y, He D (2009) Age and growth of Schizopygopsis younghusbandi younghusbandi in the Yarlung Zangbo River in Tibet, China. Environ Biol Fish 86:155–162
He D, Chen Y (2007) Molecular phylogeny and biogeography of the highly specialized grade schizothoracine fishes (Teleostei: Cyprinidae) inferred from cytochrome b sequences. Chin Sci Bull 52(6):777–788
He D, Chen Y, Chen Y, Chen Z (2004) Molecular phylogeny of the specialized grade schizothoracine fishes (Teleostei: Cyprinidae), with their implications for the uplift of the Qinghai-Tibetan Plateau. Chin Sci Bull 49(1):39–48
Julier B (2009) A program to test linkage disequilibrium between loci in autotetraploid species. Mol Ecol Resour 9:746–748
Petr T (ed.) (1999) Fish and fisheries at higher altitudes: Asia FAO Fish Tech Pap No. 385. Rome, FAO. 304 p
Qi D, Li T, Zhao X, Li J (2006) Mitochondrial cytochrome b sequence variation and phylogenetics of the highly specialized schizothoracine fishes (Teleostei: Cyprinidae) in the Qinghai-Tibet Plateau. Biochem Genet 44:270–285
Qiao H, Cheng Q, Chen Y, Ren G (2013) The complete mitochondrial genome sequence of Schizopygopsis younghusbandi (Cypriniformes: Cyprinidae). Mitochondrial DNA. doi:10.3109/19401736.2013.763240
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Techen N, Arias RS, Glynn NC (2010) Optimized construction of microsatellite-enriched. Mol Ecol Resour 10:508–515
Thrall PH, Young A (2000) AUTOTET: a program for analysis of autotetraploid genotypic data. J Hered 91:348–349
Wu Y, Kang B, Men Q (1999) Chromosome diversity of tibetan fishes. Zool Res 20:258–264
Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16
Zhang C, Cai B, Xu T (1995) Fishes and fish resources in Xizang, China. Beijing, China agriculture press, pp. 110–111 (in Chinese)
Acknowledgments
We thank Dr. Ding Chengzhi, Tao Juan and Liu chunlong for assistance in collecting samples in fieldwork. This research was supported by NSFC (41030208 and 31071900) and the Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy Of Sciences.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
He, D., Tang, W., Li, C. et al. Development of eighteen polymorphic microsatellite markers in Schizopygopsis younghusbandi Regan, 1905 (Ostariophysi: Cyprinidae) in Tibet, China. Conservation Genet Resour 5, 895–897 (2013). https://doi.org/10.1007/s12686-013-9927-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-013-9927-3