Advertisement

Conservation Genetics Resources

, Volume 5, Issue 3, pp 783–786 | Cite as

Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus Chaetodon

  • Stefano R. Montanari
  • Michael G. Gardner
  • Jean-Paul A. Hobbs
  • Morgan S. Pratchett
  • Line K. Bay
  • Lynne van Herwerden
Technical Note

Abstract

Twenty polymorphic microsatellite loci were developed via 454 sequencing for two hybridizing sister species of butterflyfish: the spot-band butterflyfish (Chaetodon punctatofasciatus) and peppered butterflyfish (Chaetodon guttatissimus), which are widely distributed in the Western Pacific and Indian Ocean, respectively. All loci were genotyped in samples collected from Christmas Island: C. guttatissimus (n = 25), C. punctatofasciatus (n = 17) and hybrids (n = 16). Mean alleles per locus (N a ) were: 9.05 for C. guttatissimus, 9.95 for C. punctatofasciatus and 9.45 for hybrids. Observed heterozygosity (H O ) ranged from 0.00 to 1.00 for C. guttatissimus; from 0.08 to 0.88 for C. punctatofasciatus; and from 0.19 to 0.94 for hybrids. Most loci conformed to Hardy–Weinberg expectations, were in linkage equilibrium, and did not contain null alleles. These markers will be useful for testing population genetic hypotheses including patterns of hybridization in this pair of butterflyfishes.

Keywords

Allopatry Butterflyfish Christmas Island Hybridization Indo-Pacific Suture zone 

Notes

Acknowledgments

The ARC CoE for Coral Reef Studies (MSP) and AIMS (SRM) financially supported this research. We thank all staff at MEEL Townsville, AGRF Brisbane, GGF Athens and Parks Australia Christmas Island.

References

  1. Allen GR, Steene R, Allen M (1998) A guide to angelfishes and butterflyfishes. Odissey Publishing, PerthGoogle Scholar
  2. Cowman PF, Bellwood DR (2011) Coral reefs as drivers of cladogenesis: expanding coral reefs, cryptic extinction events, and the development of biodiversity hotspots. J Evol Biol 24:2543–2562PubMedCrossRefGoogle Scholar
  3. Gardner MG, Fitch AJ, Bertozzi T, Lowe AJ (2011) Rise of the machines—recommendations for ecologists when using next generation sequencing for microsatellite development. Mol Ecol Resour 11:1093–1101PubMedCrossRefGoogle Scholar
  4. Hobbs J-PA, Salmond J (2008) Cohabitation of Indian and Pacific Ocean species at Christmas and Cocos (Keeling) Islands. Coral Reefs 27:933CrossRefGoogle Scholar
  5. Hobbs J-PA, Frisch AJ, Allen GR, van Herwerden L (2009) Marine hybrid hotspot at Indo-Pacific biogeographic border. Biol Lett 5:258–261PubMedCrossRefGoogle Scholar
  6. Hobbs J-PA, van Herwerden L, Pratchett MS, Allen GR (2013) Hybridisation among coral reef butterflyfishes. In: Pratchett MS, Berumen ML, Kapoor BG (eds) Biology of butterflyfish. CRC PressGoogle Scholar
  7. Jombart T (2008) Adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics 24:1403–1405PubMedCrossRefGoogle Scholar
  8. Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106PubMedCrossRefGoogle Scholar
  9. Lawton RJ, Pratchett MS, Bay LK (2010) Isolation and characterization of 29 microsatellite loci for studies of population connectivity in the butterflyfishes Chaetodon trifascialis and Chaetodon lunulatus. Conserv Genet Resour 2:209–213CrossRefGoogle Scholar
  10. Lawton RJ, Messmer V, Pratchett MS, Bay LK (2011) High gene flow across large geographic scales reduces extinction risk for a highly specialised coral feeding butterflyfish. Mol Ecol 20:3584–3598PubMedGoogle Scholar
  11. Mallet J (2005) Hybridization as an invasion of the genome. Trends Ecol Evol 20:229–237Google Scholar
  12. McMillan WO, Weigt LA, Palumbi SR (1999) Color pattern evolution, assortative mating, and genetic differentiation in brightly colored butterflyfishes (Chaetodontidae). Evolution 53:247–260CrossRefGoogle Scholar
  13. Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin J-F (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26:403–404PubMedCrossRefGoogle Scholar
  14. Meglécz E, Nève G, Biffin E, Gardner MG (2012) Breakdown of phylogenetic signal: a survey of microsatellite densities in 454 shotgun sequences from 154 non model Eukaryote species. PLoS ONE 7:e40861PubMedCrossRefGoogle Scholar
  15. Montanari SR, van Herwerden L, Pratchett MS, Hobbs J-PA, Fugedi A (2012) Reef fish hybridization: lessons learnt from butterflyfishes (genus Chaetodon). Ecol Evol 2:310–328PubMedCrossRefGoogle Scholar
  16. Rousset F (2008) Genepop’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106PubMedCrossRefGoogle Scholar
  17. Shimizu M, Kosaka N, Shimada T, Nagahata T, Iwasaki H, Nagai H, Shiba T, Emi M (2002) Universal fluorescent labeling (UFL) method for automated microsatellite analysis. DNA Res 9:173–178PubMedCrossRefGoogle Scholar
  18. van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICROCHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  19. Wang J (2004) Sibship reconstruction from genetic data with typing errors. Genetics 166:1963–1979PubMedCrossRefGoogle Scholar
  20. Yaakub SM, Bellwood DR, van Herwerden L, Walsh FM (2006) Hybridization in coral reef fishes: introgression and bi-directional gene exchange in Thalassoma (family Labridae). Mol Phylogenet Evol 40:84–100PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Stefano R. Montanari
    • 1
    • 2
    • 7
  • Michael G. Gardner
    • 3
    • 8
    • 9
  • Jean-Paul A. Hobbs
    • 4
  • Morgan S. Pratchett
    • 5
  • Line K. Bay
    • 6
  • Lynne van Herwerden
    • 2
    • 7
  1. 1.AIMS@JCUJames Cook UniversityTownsvilleAustralia
  2. 2.School of Marine and Tropical BiologyJames Cook UniversityTownsvilleAustralia
  3. 3.School of Biological SciencesFlinders UniversityAdelaideAustralia
  4. 4.The Oceans Institute and School of Plant BiologyUniversity of Western AustraliaCrawleyAustralia
  5. 5.ARC Centre of Excellence for Coral Reef StudiesJames Cook UniversityTownsvilleAustralia
  6. 6.Australian Institute of Marine ScienceTownsvilleAustralia
  7. 7.Molecular Ecology and Evolution LaboratoryJames Cook UniversityTownsvilleAustralia
  8. 8.Australian Centre for Evolutionary Biology and BiodiversityUniversity of AdelaideAdelaideAustralia
  9. 9.Evolutionary Biology UnitSouth Australian MuseumNorth Terrace AdelaideAustralia

Personalised recommendations