Conservation Genetics Resources

, Volume 5, Issue 3, pp 783–786 | Cite as

Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus Chaetodon

  • Stefano R. Montanari
  • Michael G. Gardner
  • Jean-Paul A. Hobbs
  • Morgan S. Pratchett
  • Line K. Bay
  • Lynne van Herwerden
Technical Note


Twenty polymorphic microsatellite loci were developed via 454 sequencing for two hybridizing sister species of butterflyfish: the spot-band butterflyfish (Chaetodon punctatofasciatus) and peppered butterflyfish (Chaetodon guttatissimus), which are widely distributed in the Western Pacific and Indian Ocean, respectively. All loci were genotyped in samples collected from Christmas Island: C. guttatissimus (n = 25), C. punctatofasciatus (n = 17) and hybrids (n = 16). Mean alleles per locus (N a ) were: 9.05 for C. guttatissimus, 9.95 for C. punctatofasciatus and 9.45 for hybrids. Observed heterozygosity (H O ) ranged from 0.00 to 1.00 for C. guttatissimus; from 0.08 to 0.88 for C. punctatofasciatus; and from 0.19 to 0.94 for hybrids. Most loci conformed to Hardy–Weinberg expectations, were in linkage equilibrium, and did not contain null alleles. These markers will be useful for testing population genetic hypotheses including patterns of hybridization in this pair of butterflyfishes.


Allopatry Butterflyfish Christmas Island Hybridization Indo-Pacific Suture zone 



The ARC CoE for Coral Reef Studies (MSP) and AIMS (SRM) financially supported this research. We thank all staff at MEEL Townsville, AGRF Brisbane, GGF Athens and Parks Australia Christmas Island.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Stefano R. Montanari
    • 1
    • 2
    • 7
  • Michael G. Gardner
    • 3
    • 8
    • 9
  • Jean-Paul A. Hobbs
    • 4
  • Morgan S. Pratchett
    • 5
  • Line K. Bay
    • 6
  • Lynne van Herwerden
    • 2
    • 7
  1. 1.AIMS@JCUJames Cook UniversityTownsvilleAustralia
  2. 2.School of Marine and Tropical BiologyJames Cook UniversityTownsvilleAustralia
  3. 3.School of Biological SciencesFlinders UniversityAdelaideAustralia
  4. 4.The Oceans Institute and School of Plant BiologyUniversity of Western AustraliaCrawleyAustralia
  5. 5.ARC Centre of Excellence for Coral Reef StudiesJames Cook UniversityTownsvilleAustralia
  6. 6.Australian Institute of Marine ScienceTownsvilleAustralia
  7. 7.Molecular Ecology and Evolution LaboratoryJames Cook UniversityTownsvilleAustralia
  8. 8.Australian Centre for Evolutionary Biology and BiodiversityUniversity of AdelaideAdelaideAustralia
  9. 9.Evolutionary Biology UnitSouth Australian MuseumNorth Terrace AdelaideAustralia

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