Abstract
We isolated and characterized 21 microsatellite loci in the vulnerable and iconic Australian lungfish, Neoceratodus forsteri. Loci were screened across eight individuals from the Burnett River and 40 individuals from the Pine River. Genetic diversity was low with between one and six alleles per locus within populations and a maximum expected heterozygosity of 0.774. These loci will now be available to assess effective population sizes and genetic structure in N. forsteri across its natural range in South East Queensland, Australia.
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Acknowledgments
This microsatellite library was developed with funding assistance from Seqwater. Tinana and Burnett samples were collected under General Fisheries Permit 157 882, and AEC approvals CA2010/10/473 and CA2011/07/522. Pine River samples were collected under General Fisheries Permit No. 140615 and AEC approval CA2011/10/551.
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Huey, J.A., Real, K.M., Mather, P.B. et al. Isolation and characterization of 21 polymorphic microsatellite loci in the iconic Australian lungfish, Neoceratodus forsteri, using the Ion Torrent next-generation sequencing platform. Conservation Genet Resour 5, 737–740 (2013). https://doi.org/10.1007/s12686-013-9894-8
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DOI: https://doi.org/10.1007/s12686-013-9894-8