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Isolation and characterization of eighteen microsatellite loci for the largemouth bass, Micropterus salmoides, and cross amplification in congeneric species

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Abstract

We used a PCR-based technique to isolate 18 microsatellite loci for the largemouth bass, Micropterus salmoides. These loci were characterized using sampled populations in Florida and Wisconsin. The mean number of alleles at a locus was 8 for the sample from Florida and 5 for the samples from Wisconsin. The observed heterozygosities were 0.57 and 0.33 for the samples from Florida and Wisconsin, respectively. There was evidence of genetic differentiation between the Florida and Wisconsin samples at all 18 loci, indicating spatial structure within the range of M. salmoides. The markers cross-amplified in the seven other micropterid species, which suggests they would have utility for studies of hybridization among other members of the genus.

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Acknowledgments

This work was supported financially by the State of Florida, Mote Marine Laboratory, the Wildlife Foundation of Florida’s Conserve Wildlife Tag Grant (CWT 0607-05), and the U.S. Geological Survey. We would like to thank the following individuals for collecting the specimens included in this study: Dijar Lutz-Carillo (Texas Parks and Wildlife Department), T. Ivasauskas (Tennessee Tech University), Steve Sammons (Auburn University), David Philipp (Illinois Natural History Survey), Jean Leitner (South Carolina Department of Natural Resources).

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Authors and Affiliations

  1. Florida Fish and Wildlife Research Institute, 100 Eighth Avenue S.E., Saint Petersburg, FL, 33701-5095, USA

    Seifu Seyoum, Brandon L. Barthel, Michael D. Tringali, Michelle C. Davis, Samantha L. Schmitt & Pam S. Bellotti

  2. Florida Fish and Wildlife Research Institute, 601 W. Woodward Ave., Eustis, FL, 32726, USA

    Michael D. Tringali & Wesley F. Porak

Authors
  1. Seifu Seyoum
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  2. Brandon L. Barthel
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  3. Michael D. Tringali
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  4. Michelle C. Davis
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  5. Samantha L. Schmitt
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  6. Pam S. Bellotti
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  7. Wesley F. Porak
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Correspondence to Michael D. Tringali.

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Seyoum, S., Barthel, B.L., Tringali, M.D. et al. Isolation and characterization of eighteen microsatellite loci for the largemouth bass, Micropterus salmoides, and cross amplification in congeneric species. Conservation Genet Resour 5, 697–701 (2013). https://doi.org/10.1007/s12686-013-9885-9

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  • DOI: https://doi.org/10.1007/s12686-013-9885-9

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