Characterization of 10 polymorphic microsatellite loci in Munidopsis lauensis, a squat-lobster from the southwestern Pacific

Abstract

Munidopsis lauensis is a deep-sea squat lobster commonly associated with hydrothermal vents at southwest Pacific back-arc spreading centers. Eight selectively neutral and unlinked polymorphic microsatellite loci were developed for this species and two additional loci from a related species were successfully cross-amplified. Eight of the ten total loci conformed to Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.23 to 0.64 (mean H O  = 0.50, SD = 0.15). Seven of ten loci cross-amplified in Munidopsis antonii, a closely related species. Microsatellites developed for M. lauensis are being deployed to study connectivity among populations of this species that occur at hydrothermal vents in Manus and Lau Basins.

This is a preview of subscription content, log in to check access.

References

  1. Antao T, Lopes RJ, Beja-Pereira A, Luikart G (2008) LOSITAN: a workbench to detect molecular adaptation based on a Fst-outlier method. BMC Bioinformatics 9:323

    PubMed  Article  Google Scholar 

  2. Baba K, de Saint Laurent M (1992) Chirostylid and galatheid crustaceans (Decapoda: Anomura) from active thermal vent areas in the SW Pacific. Sci Mar 56:321–332

    Google Scholar 

  3. Bailie DA, Hynes R, Prodöhl PA (2011) Genetic parentage in the squat lobsters Munida rugosa and M. sarsi (Crustacea, Anomura, Galatheidae). Mar Ecol Prog Ser 421:173–182

    Article  Google Scholar 

  4. Beaumont MA, Nicholas RA (1996) Evaluating loci for use in the genetic analysis of population structure. Proc R Soc Lond B 263:1619–1626

    Article  Google Scholar 

  5. Cabezas P, Bloor P, Acevedo I, Toledo C, Calvo M, Macpherson E, Machordom A (2009) Development and characterization of microsatellite markers for the endangered anchialine squat lobster Munidopsis polymorpha. Conserv Genet 10:673–676

    Article  CAS  Google Scholar 

  6. Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94

    PubMed  Article  CAS  Google Scholar 

  7. Kalinowski ST (2006) HW-QuickCheck: an easy-to-use computer program for checking genotypes for agreement with Hardy–Weinberg expectations. Mol Ecol Notes 6:974–979

    Article  Google Scholar 

  8. Rice WR (1989) Analyzing tables of statistical tests. Evolution 43(1):223–225

    Article  Google Scholar 

  9. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa

    Google Scholar 

  10. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234

    PubMed  Article  CAS  Google Scholar 

  11. Schultz T, Hsing P, Eng A, Zelnio K (2010) Characterization of 18 polymorphic microsatellite loci from Bathymodiolus manusensis (Bivalvia, Mytilidae) from deep-sea hydrothermal vents. Conserv Genet Resour 3:25–27

    Article  Google Scholar 

  12. Thaler AD, Zelnio K, Jones R, Carlsson J, Van Dover CL, Schultz TF (2010) Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents. Conserv Genet Resour 2:101–103

    Article  Google Scholar 

  13. Thaler AD, Zelnio K, Saleu W, Schultz TF, Carlsson J, Cunningham C, Vrijenhoek RC, Van Dover CL (2011) The spatial scale of genetic subdivision in populations of Ifremeria nautilei, a hydrothermal-vent gastropod from the southwest Pacific. BMC Evol Biol 11(1):372

    PubMed  Article  Google Scholar 

  14. van Oosterhout C, Hutchinson W, Willds D, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

  15. Vrijenhoek RC (1997) Gene flow and genetic diversity in naturally fragmented metapopulations of deep-sea hydrothermal vent animals. J Hered 88(4):285–293

    Google Scholar 

  16. Vrijenhoek RC (2010) Genetic diversity and connectivity of deep sea hydrothermal vent metapopulations. Mol Ecol 19(20):4391–4411

    Google Scholar 

  17. Zelnio KA, Thaler AD, Jones RE, Saleu W, Schultz TF, Van Dover CL, Carlsson J (2010) Characterization of nine polymorphic microsatellite loci in Chorocaris sp. (Crustacea, Caridea, Alvinocarididae) from deep-sea hydrothermal vents. Conserv Genet Resour 2(1):223–226

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported by a research contract from Nautilus Minerals Niugini Limited to CLVD. The authors thank Dr. Samantha Smith of Nautilus Minerals, the captain and crew of the M/V Nor Sky, the Canyon Offshore ROV team, Rebecca Jones and Pen-Yuan Hsing for assistance with field sampling, and Freddie Alei and Bernard Ball for assistance with bench work. Munidopsis lauensis samples from Manus Basin are the property of Papua New Guinea, are held in trust by Nautilus Minerals, and on loan to Duke University for baseline studies for the Solwara 1 Project. ADT, SP, CLVD conceived the study; ADT collected samples; EAB, AJ undertook molecular bench work; EAB, ADT, AJ, SP analyzed the data; EAB, ADT, AJ, SP, CLVD drafted the manuscript. All authors have read and approved the final manuscript.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Andrew David Thaler.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Boyle, E.A., Thaler, A.D., Jacobson, A. et al. Characterization of 10 polymorphic microsatellite loci in Munidopsis lauensis, a squat-lobster from the southwestern Pacific. Conservation Genet Resour 5, 647–649 (2013). https://doi.org/10.1007/s12686-013-9872-1

Download citation

Keywords

  • Munidopsis antonii
  • Hydrothermal vent
  • Back-arc basin
  • Manus Basin