Munidopsis lauensis is a deep-sea squat lobster commonly associated with hydrothermal vents at southwest Pacific back-arc spreading centers. Eight selectively neutral and unlinked polymorphic microsatellite loci were developed for this species and two additional loci from a related species were successfully cross-amplified. Eight of the ten total loci conformed to Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.23 to 0.64 (mean H O = 0.50, SD = 0.15). Seven of ten loci cross-amplified in Munidopsis antonii, a closely related species. Microsatellites developed for M. lauensis are being deployed to study connectivity among populations of this species that occur at hydrothermal vents in Manus and Lau Basins.
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Antao T, Lopes RJ, Beja-Pereira A, Luikart G (2008) LOSITAN: a workbench to detect molecular adaptation based on a Fst-outlier method. BMC Bioinformatics 9:323
Baba K, de Saint Laurent M (1992) Chirostylid and galatheid crustaceans (Decapoda: Anomura) from active thermal vent areas in the SW Pacific. Sci Mar 56:321–332
Bailie DA, Hynes R, Prodöhl PA (2011) Genetic parentage in the squat lobsters Munida rugosa and M. sarsi (Crustacea, Anomura, Galatheidae). Mar Ecol Prog Ser 421:173–182
Beaumont MA, Nicholas RA (1996) Evaluating loci for use in the genetic analysis of population structure. Proc R Soc Lond B 263:1619–1626
Cabezas P, Bloor P, Acevedo I, Toledo C, Calvo M, Macpherson E, Machordom A (2009) Development and characterization of microsatellite markers for the endangered anchialine squat lobster Munidopsis polymorpha. Conserv Genet 10:673–676
Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94
Kalinowski ST (2006) HW-QuickCheck: an easy-to-use computer program for checking genotypes for agreement with Hardy–Weinberg expectations. Mol Ecol Notes 6:974–979
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43(1):223–225
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234
Schultz T, Hsing P, Eng A, Zelnio K (2010) Characterization of 18 polymorphic microsatellite loci from Bathymodiolus manusensis (Bivalvia, Mytilidae) from deep-sea hydrothermal vents. Conserv Genet Resour 3:25–27
Thaler AD, Zelnio K, Jones R, Carlsson J, Van Dover CL, Schultz TF (2010) Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents. Conserv Genet Resour 2:101–103
Thaler AD, Zelnio K, Saleu W, Schultz TF, Carlsson J, Cunningham C, Vrijenhoek RC, Van Dover CL (2011) The spatial scale of genetic subdivision in populations of Ifremeria nautilei, a hydrothermal-vent gastropod from the southwest Pacific. BMC Evol Biol 11(1):372
van Oosterhout C, Hutchinson W, Willds D, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Vrijenhoek RC (1997) Gene flow and genetic diversity in naturally fragmented metapopulations of deep-sea hydrothermal vent animals. J Hered 88(4):285–293
Vrijenhoek RC (2010) Genetic diversity and connectivity of deep sea hydrothermal vent metapopulations. Mol Ecol 19(20):4391–4411
Zelnio KA, Thaler AD, Jones RE, Saleu W, Schultz TF, Van Dover CL, Carlsson J (2010) Characterization of nine polymorphic microsatellite loci in Chorocaris sp. (Crustacea, Caridea, Alvinocarididae) from deep-sea hydrothermal vents. Conserv Genet Resour 2(1):223–226
This research was supported by a research contract from Nautilus Minerals Niugini Limited to CLVD. The authors thank Dr. Samantha Smith of Nautilus Minerals, the captain and crew of the M/V Nor Sky, the Canyon Offshore ROV team, Rebecca Jones and Pen-Yuan Hsing for assistance with field sampling, and Freddie Alei and Bernard Ball for assistance with bench work. Munidopsis lauensis samples from Manus Basin are the property of Papua New Guinea, are held in trust by Nautilus Minerals, and on loan to Duke University for baseline studies for the Solwara 1 Project. ADT, SP, CLVD conceived the study; ADT collected samples; EAB, AJ undertook molecular bench work; EAB, ADT, AJ, SP analyzed the data; EAB, ADT, AJ, SP, CLVD drafted the manuscript. All authors have read and approved the final manuscript.
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Boyle, E.A., Thaler, A.D., Jacobson, A. et al. Characterization of 10 polymorphic microsatellite loci in Munidopsis lauensis, a squat-lobster from the southwestern Pacific. Conservation Genet Resour 5, 647–649 (2013). https://doi.org/10.1007/s12686-013-9872-1
- Munidopsis antonii
- Hydrothermal vent
- Back-arc basin
- Manus Basin