Abstract
Munidopsis lauensis is a deep-sea squat lobster commonly associated with hydrothermal vents at southwest Pacific back-arc spreading centers. Eight selectively neutral and unlinked polymorphic microsatellite loci were developed for this species and two additional loci from a related species were successfully cross-amplified. Eight of the ten total loci conformed to Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.23 to 0.64 (mean H O = 0.50, SD = 0.15). Seven of ten loci cross-amplified in Munidopsis antonii, a closely related species. Microsatellites developed for M. lauensis are being deployed to study connectivity among populations of this species that occur at hydrothermal vents in Manus and Lau Basins.
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Acknowledgments
This research was supported by a research contract from Nautilus Minerals Niugini Limited to CLVD. The authors thank Dr. Samantha Smith of Nautilus Minerals, the captain and crew of the M/V Nor Sky, the Canyon Offshore ROV team, Rebecca Jones and Pen-Yuan Hsing for assistance with field sampling, and Freddie Alei and Bernard Ball for assistance with bench work. Munidopsis lauensis samples from Manus Basin are the property of Papua New Guinea, are held in trust by Nautilus Minerals, and on loan to Duke University for baseline studies for the Solwara 1 Project. ADT, SP, CLVD conceived the study; ADT collected samples; EAB, AJ undertook molecular bench work; EAB, ADT, AJ, SP analyzed the data; EAB, ADT, AJ, SP, CLVD drafted the manuscript. All authors have read and approved the final manuscript.
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Boyle, E.A., Thaler, A.D., Jacobson, A. et al. Characterization of 10 polymorphic microsatellite loci in Munidopsis lauensis, a squat-lobster from the southwestern Pacific. Conservation Genet Resour 5, 647–649 (2013). https://doi.org/10.1007/s12686-013-9872-1
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DOI: https://doi.org/10.1007/s12686-013-9872-1