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Development and characterization of 15 polymorphic microsatellite markers for North Pacific albatrosses using paired-end Illumina shotgun sequencing

  • Microsatellite Letters
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Abstract

All three species of North Pacific albatross are vulnerable or near threatened (IUCN 2013) yet microsatellite markers to study their population characteristics are lacking. We employed 250-bp paired-end whole genome shotgun sequencing on an Illumina MiSeq platform to discover microsatellite markers. We selected 16 candidate loci for screening in 36 Black-footed (Phoebastria nigripes), 12 Laysan (P. immutabilis), and 3 Short-tailed (P. albatrus) albatrosses from which we characterized 15 markers.

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Acknowledgments

We thank Hannahrose Nevins and Jesse Beck of the Moss Landing Marine Laboratories for contributing tissue samples from fisheries by-catch and stranded albatrosses.

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Authors and Affiliations

  1. Mammalian Ecology and Conservation Unit, Veterinary Genetics Laboratory, University of California, Davis, One Shields Avenue/Old Davis Rd., Davis, CA, 95616, USA

    Luis D. Hernandez, Zachary T. Lounsberry & Benjamin N. Sacks

  2. Santa Barbara Museum of Natural History, 2559 Puesta Del Sol, Santa Barbara, CA, 93105, USA

    Paul W. Collins

  3. Long Marine Laboratory, Center for Ocean Health, Institute of Marine Sciences, University of California, Santa Cruz, 100 Shaffer Rd, Santa Cruz, CA, 95060, USA

    Robert W. Henry

  4. Department of Biology, University of New Mexico, Albuquerque, NM, 87131, USA

    Seth D. Newsome

  5. Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA

    Benjamin N. Sacks

Authors
  1. Luis D. Hernandez
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  2. Zachary T. Lounsberry
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  3. Paul W. Collins
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  4. Robert W. Henry
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  5. Seth D. Newsome
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  6. Benjamin N. Sacks
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Corresponding author

Correspondence to Benjamin N. Sacks.

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Hernandez, L.D., Lounsberry, Z.T., Collins, P.W. et al. Development and characterization of 15 polymorphic microsatellite markers for North Pacific albatrosses using paired-end Illumina shotgun sequencing. Conservation Genet Resour 6, 491–493 (2014). https://doi.org/10.1007/s12686-013-0138-8

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  • DOI: https://doi.org/10.1007/s12686-013-0138-8

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