Conservation Genetics Resources

, Volume 6, Issue 2, pp 465–468 | Cite as

Characterization of microsatellite loci from two-spotted octopus Octopus bimaculatus Verrill 1883 from pyrosequencing reads

  • J. F. Domínguez-Contreras
  • A. Munguía-Vega
  • B. P. Ceballos-Vázquez
  • M. Arellano-Martínez
  • M. Culver
Microsatellite Letters

Abstract

We characterized 22 novel microsatellite loci in the two-spotted octopus Octopus bimaculatus using 454 pyrosequencing reads. All loci were polymorphic and will be used in studies of marine connectivity aimed at increasing sustainability of the resource. The mean number alleles per locus was 13.09 (range 7–19) and observed heterozygosities ranged from 0.50 to 1.00. Four loci pairs were linked and three deviated from Hardy–Weinberg equilibrium. Eighteen and 12 loci were polymorphic in Octopus bimaculoides and Octopus hubbsorum, respectively.

Keywords

Marine connectivity Gulf of California Octopus Mexican Pacific Next-generation sequencing 

References

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • J. F. Domínguez-Contreras
    • 1
  • A. Munguía-Vega
    • 2
    • 3
  • B. P. Ceballos-Vázquez
    • 1
  • M. Arellano-Martínez
    • 1
  • M. Culver
    • 4
  1. 1.Instituto Politécnico Nacional CICIMARLa PazMexico
  2. 2.Conservation Genetics Laboratory, School of Natural Resources and Environment, BSE-317University of ArizonaTucsonUSA
  3. 3.Comunidad y Biodiversidad A.C.GuaymasMexico
  4. 4.Conservation Genetics Laboratory, Arizona Cooperative Fish and Wildlife Research Unit, School of Natural Resources and Environment, U.S. Geological Survey, BSE-317University of ArizonaTucsonUSA

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