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Isolation and characterization of 8 polymorphic microsatellite markers from the Greater Short-horned Lizard (Phrynosoma hernandesi)

  • Microsatellite Letters
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Abstract

The Greater Short-horned Lizard (Phrynosoma hernandesi) is a small reptile endemic to western North America that is classified as endangered in Canada. Here, we describe the development of 8 microsatellite markers for P. hernandesi. Observed allelic diversity varied from 2 to 11 while observed heterozygosity and expected heterozygosity ranged from 0.370–0.852 to 0.486–0.856, respectively. These markers will facilitate studies that address conservation of the species, such as levels of genetic diversity and population structure.

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References

  • COSEWIC (2007) COSEWIC assessment and update status report on the Greater Short-horned Lizard Phrynosoma hernandesi in Canada. Committee on the Status of Endangered Wildlife in Canada, Ottawa, pp vi + 41

  • Hamilton MB, Pincus EL, DiFiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27:500–507

    CAS  PubMed  Google Scholar 

  • Rousset F (2008) GENEPOP’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–110

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–396

    Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This study was conducted as part of BIOL 592 at the University of Alberta. We thank all participants in this course and the Department of Biological Sciences Molecular Biology Facility. Special thanks to members of the field team that collected samples used in this study. Specimens were collected under the authority of Alberta Fish and Wildlife collection permit #39496, and Alberta Fish and Wildlife research permit #39552. Sampling of tail tips for the retrieval of microsatellite markers was conducted under the auspices of University of Calgary Animal Care permit BI 2009-33.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Alberta, 116 St and 85 Ave, Edmonton, AB, T6G 2R3, Canada

    Zijian Sim, Brandon K. Booker, Michelle Viengkone, Corey S. Davis & Cynthia A. Paszkowski

  2. Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada

    Magdalene N.-Y. Leung & Anthony P. Russell

Authors
  1. Zijian Sim
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  2. Brandon K. Booker
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  3. Michelle Viengkone
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  4. Corey S. Davis
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  5. Magdalene N.-Y. Leung
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  6. Anthony P. Russell
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  7. Cynthia A. Paszkowski
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Correspondence to Zijian Sim.

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Sim, Z., Booker, B.K., Viengkone, M. et al. Isolation and characterization of 8 polymorphic microsatellite markers from the Greater Short-horned Lizard (Phrynosoma hernandesi). Conservation Genet Resour 6, 443–444 (2014). https://doi.org/10.1007/s12686-013-0119-y

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  • DOI: https://doi.org/10.1007/s12686-013-0119-y

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