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Characterisation of microsatellite DNA markers for the Wiry Honey Myrtle, Melaleuca nematophylla Craven

  • Microsatellite Letters
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Abstract

A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Melaleuca nematophylla, the Wiry Honey Myrtle, a common shrub of southwest Western Australia. Populations are patchily distributed in a highly fragmented landscape due to extensive vegetation clearing for agriculture and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Moderate diversity was observed in two populations with the number of alleles per locus ranging from 2 to 17. Expected and observed heterozygosity averaged 0.606 and 0.423, respectively. All loci showed independent inheritance but there was evidence of possible null alleles at some loci in each population.

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Acknowledgments

Funding was provided by the Australian Research Council and Karara Mining Limited. We thank David Beven for assistance with field collections and Peter Nguyen of the Botanic Gardens and Parks Authority for provision of original genetic material.

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Authors and Affiliations

  1. Science and Conservation Division, Department of Parks and Wildlife, Locked Bag 104, Bentley Delivery Centre, Bentley, WA, 6983, Australia

    Melissa A. Millar, Margaret Byrne & David J. Coates

  2. School of Animal Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    Melissa A. Millar

  3. School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    Margaret Byrne & David J. Coates

  4. School of Animal Biology and Centre of Excellence in Natural Resource Management, The University of Western Australia, PO Box 5771, Albany, WA, 6332, Australia

    J. Dale Roberts

Authors
  1. Melissa A. Millar
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  2. Margaret Byrne
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  3. David J. Coates
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  4. J. Dale Roberts
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Corresponding author

Correspondence to Melissa A. Millar.

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Millar, M.A., Byrne, M., Coates, D.J. et al. Characterisation of microsatellite DNA markers for the Wiry Honey Myrtle, Melaleuca nematophylla Craven. Conservation Genet Resour 6, 439–441 (2014). https://doi.org/10.1007/s12686-013-0118-z

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  • DOI: https://doi.org/10.1007/s12686-013-0118-z

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