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Development of microsatellite markers for Peristenus digoneutis (Hymenoptera: Braconidae), a key natural enemy of tarnished plant bugs

  • Microsatellite Letters
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Abstract

The introduced European parasitoid Peristenus digoneutis is a key biocontrol agent of lygus bug pests in agricultural crops in the Northeastern USA. Changes in cultural practices and reduction of alfalfa culture have significantly reduced its abundance in its native range from historic levels. Of 48 microsatellite loci isolated from P. digoneutis genome using 454 pyrosequencing, 24 were retained based upon consistent amplification. Sixteen loci exhibited polymorphism in populations sampled in France and the USA. The number of alleles ranged from 2 to 9. The observed heterozygosity (H o) and the expected heterozygosity (H e) varied from 0.043 to 0.710 and from 0.103 to 0.728, respectively. Most loci conformed to Hardy–Weinberg equilibrium with the exclusion of five loci. These polymorphic loci will be valuable for population genetic studies and genetic conservation for P. digoneutis.

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References

  • Day WH (1996) Evaluation of biological control of the tarnished plant bug (Hemiptera: Miridae) in alfalfa by the introduced parasite Peristenus digoneutis (Hymenoptera: Braconidae). Environ Entomol 25:512–518

    Google Scholar 

  • Lepais O, Bacles CFE (2011) De novo discovery and multiplexed amplification of microsatellite markers for black alder (Alnus glutinosa) and related species using SSR-enriched shotgun pyrosequencing. J Hered 102:622–626. doi:10.1093/jhered/esr062

    Article  Google Scholar 

  • Malausa T, Gilles A, Meglécz E, Ferreira S, Pech N et al (2011) High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. Mol Ecol Resour 11:638–644. doi:10.1111/j.1755-0998.2011.02992.x

    Article  CAS  PubMed  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): a population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Schellhorn NA, Silberbauer LX (2003) The role of surrounding vegetation and refuges: increasing the effectiveness of natural enemies in cotton and broccoli systems. In: Proceedings for the 1st international symposium on the biological control of arthropods, Honolulu, Hawaii, USA, pp 235–243

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Acknowledgments

We thank Frederique Cerqueira (Plateforme Génomique Environnementale du Labex, Montpellier) for technical help. V. Marcari was the recipient of a scholarship from University of Rome.

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Authors and Affiliations

  1. USDA-ARS European Biological Control Laboratory (EBCL), 810 Avenue du Campus d’Agropolis, 34980, Montferrier sur Lez, France

    Veronica Marcari, Sandrine Causse, Kim Hoelmer, Dominique Coutinot & Marie-Claude Bon

  2. Department of Biology and Biotechnologies “Charles Darwin”, Sapienza Rome University, Viale Università 32, 00185, Rome, Italy

    Veronica Marcari & Alessio De Biase

Authors
  1. Veronica Marcari
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  2. Sandrine Causse
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  3. Kim Hoelmer
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  4. Dominique Coutinot
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  5. Alessio De Biase
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  6. Marie-Claude Bon
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Corresponding author

Correspondence to Marie-Claude Bon.

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Marcari, V., Causse, S., Hoelmer, K. et al. Development of microsatellite markers for Peristenus digoneutis (Hymenoptera: Braconidae), a key natural enemy of tarnished plant bugs. Conservation Genet Resour 6, 421–423 (2014). https://doi.org/10.1007/s12686-013-0108-1

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  • DOI: https://doi.org/10.1007/s12686-013-0108-1

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