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Isolation and characterization of polymorphic microsatellite loci in the endangered San Diego fairy shrimp (Branchinecta sandiegonensis)

  • Microsatellite Letters
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Abstract

A set of ten polymorphic microsatellite loci were isolated and characterized for the endangered San Diego fairy shrimp (Branchinecta sandiegonensis). These loci were amplified on a set of 24 fairy shrimp collected from vernal pools located throughout San Diego County. The loci selected are highly variable across a wide sampling range (3–29 alleles per locus, 3–20 heterozygotes observed). After directly testing for Mendelian inheritance through family screens, eight markers did not show evidence of null alleles. This novel set of microsatellite markers will be useful in future genetic studies to assess intraspecific diversity, population connectivity and mating patterns.

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References

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Acknowledgments

This project was funded by the San Diego Association of Governments (SANDAG). We thank Genetic Identification Services for providing the library of B. sandiegonensis microsatellite markers; and Eton Biosciences for their genotyping services, without which this project would not have been possible. We also thank Dr. Rulon Clark and Dr. Li An (SDSU) for their assistance and advice regarding this project. All “take” was conducted under the supervision of Simovich as authorized under US Fish and Wildlife Service Permit TE-787037-4.

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Authors and Affiliations

  1. Department of Biology, San Diego State University, 5500 Campanile Dr., San Diego, CA, 92182-4614, USA

    Joelle M. Andrews & Andrew J. Bohonak

  2. Department of Biology, University of San Diego, 5998 Alcalá Park, San Diego, CA, 92110, USA

    Marie A. Simovich

Authors
  1. Joelle M. Andrews
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  2. Andrew J. Bohonak
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  3. Marie A. Simovich
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Correspondence to Joelle M. Andrews.

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Andrews, J.M., Bohonak, A.J. & Simovich, M.A. Isolation and characterization of polymorphic microsatellite loci in the endangered San Diego fairy shrimp (Branchinecta sandiegonensis). Conservation Genet Resour 6, 401–403 (2014). https://doi.org/10.1007/s12686-013-0103-6

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  • DOI: https://doi.org/10.1007/s12686-013-0103-6

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